Acetobacter pasteurianus IFO 13751의 돌연변이주에 의한 다당류 생산성

Productivity of Polysaccharide by Mutant of Acetobacter pasteurianus IFO 13751

초산균의 변이주 중 다당류 생산량이 우수한 균수를 선별한 결과 Acetobacter pasteurianus IFO 13751-5가 친주보다 약 3배의 다당류 생산량을 나타내었다. 돌연변이주인 Acetobacter pasteurianus IFO 13751-5의 최적 배양조건을 검토한 결과 탄소원은 sucrose로써 5% 첨가시 45.95 mg/ml로 가장 높게 나타났다. 다당류를 대량생산할 목적으로 jar fermentor를 이용하여 생산된 다당류는 72시간에서 55.10 mg/ml의 최대 생산량을 나타내었다. 이 배양액을 분리 정제하여 진공 동결건조한 다당류는 18.93 g/l을 얻었다.

In order to obtain the highest productivity of polysaccharide, acetic acid bacteria was used. Several acetic acid bacteria were investigated to the productivity of polysaccharide, an mutant, Acetobacter pasteurianus IFO 13751-5 selected among serveral acetic acid bacteria which can produce the polysaccharide by radiation of ultra-violet ray. Acetobacter pasteurianus IFO 13751-5 was shown 3 fold polysaccharide production than that of its parents. When the Acetobacter pasteurianus IFO 13751-5 was investigated under the condition of carbon source containing 5% sucrose, the highest amount of polysaccharide (45.95 mg/ml) was obtained. The polysaccharide production by Acetobacter pasteurianus IFO 13751-5 was 55.10 mg/ml by using jar fermentor.