Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Lactic Acid, Ethylalcohol and 4-Ethylguaiacol Contents of Rapid Fermentation of Sardine Soy Sauce Prepared by Using Immobilized Whole Cells

고정화균체를 이용하여 속성 발효시킨 정어리 어간장의 젖산, 알코올 및 4-ethylguaiacol의 함량

  • Ryu, Beung-Ho (Department of Food Science and Technology, Kyungsung University) ;
  • Kim, Seong-Joon (Public Health Environment Institute of Pusan) ;
  • Shin, Dong-Bun (Department of Food Science and Technology, Kyungsung University)
  • 류병호 (경성대학교 식품공학과) ;
  • 김성준 (부산시 보건환경연구원) ;
  • 신동분 (경성대학교 식품공학과)
  • Published : 1992.10.01
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Abstract

This study was performed to rapid fermentation from sardine hydrolyzate by using column reactor. The column reactor was constructed from three glass columns $(30cm{\times}5cm)$ and each column was packed with colloidal silica and sodium alginate (1:5) on which Pediococcus halophilus R-22, Saccharomyces rouxii R-60 and Candida etchellsii H-50, respectively, was previously fixed. At that time, optimal conditions for rapid fermentation were found the pH of 5.2, temperature of $30^{\circ}C$ and 10% NaCl. For rapid fermentation, immobilized whole cells of P. halophilus R-22, S. ruoxii R-60 and C. etchellsii H-50 packed the each column reactor were produced 0.75% lactic acid, 2.5% ethylalcohol and 18 mg/l 4-ethylguaiacol under the optimal conditions.

본 연구는 간장의 속성발효를 위한 방법의 하나로 정어리육을 효소에 의하여 분해하고, 분해액을 유산균과 효모의 균체를 고정화하여 이를 column형 reactor를 이용하여 속성발효를 시도하였다. 이때 사용된 column 반응조는 유리제$(30{\times}50cm)$의 칼럼 3개에 Pediococcus halophilus R-22, Saccharomyces rouxii R-60과 Candida etchellsii H-50을 colloidal silic와 sodium alginate(1 : 5)의 혼합액으로 고정화하여 각각의 column에 충전시켰다. 이때의 최적조건은 pH5.2, 온도 $30^{\circ}C$, 식염농도 10%이었다. 발효 최적조건에서 96시간 경과시 P. halophilus R-22는 유산을 0.75%, S. rouxii R-60은 ethylalcohol을 2.5%, C. etchellsii H-50은 4-ethylguaiacol을 18 mg/l 생성하였다.

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