Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Resistance of Some Metal Ions on Growth of Serratia marcescens Strain P

Serratia marcescens Strain P 성장에 미치는 중금속 내성

  • Published : 1992.12.01
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Abstract

The resistant effect of several heavy metal ions to Serratia marcescens strain P was studied by the method of minimal inhibitory concentration(MIC), and testing for their metal biosorption. S. marcescens strain P showed a good survival in the presence of high concentrations of some metal ions, namely cadmium, lead, iron, magnesium, and manganese. Copper had the most inhibitory effect among tested. The MIC value was ranged from 0.79 to 1.58 mM. Cells of S. marcescens strain P exhibit an abnormally long lag phase when incubated in high concentrations of zinc and cadmium. Pigment production was reduced by zinc and cadmium, but enhanced by lead and iron. S. marcescens strain P was resistant to ampicillin, tetracycline, cefamandole and chloramphenicol with minimal inhibitory concentration of 128 $\mu$g/ml, 32 $\mu$g/ml, 256 $\mu$g/ml, and 8 $\mu$g/ml, respectively. The kinetics study of biosorptive uptake by S. marcescens strain P revealed that 16.59% of cadmium and 35.38% of lead were eliminated from the media.

공장 폐수를 비롯한 중금속 오염 환경으로부터 효율적인 중금속 제거를 위해 생물학적 오염제거에 관한 연구가 많이 이루어지고 있다. 본 실험에서는 항생제 내성 유전자를 많이 가지고 있는 것으로 알려진 Serratia marcescens를 대상으로 이 균주가 중금속들에 대해 어떤 영향을 보이는지 조사하였다. 그 결과 lead, iron, magnesium, manganese 등의 처리군에서 24시간내에 1,000ppm 이상의 고농도 처리군에서 최소억제성장농도(MIC)를 나타냈으며, cadmium에서는 600ppm 처리군에서, 구리는 800ppm의 처리군에서 MIC를 보였으며, 아연 처리군에서는 800ppm에서 MIC를 나타냈다. 또한 48시간 배양에 따른 MIC 비교 결과, 중금속의 고농도 처리군에서 매우 긴 적응기를 갖는 것으로 확인하였다. 15가지의 항생체를 대상으로 저항성을 조사한 결과, ampicillin, tetracycline, cefamandole, cephalothin에서 저항성을 보엿으며 다른 S. marcescens 균주들에 비해 chloramphenicol에 대한 특이한 민감성을 보였다. 카드뮴과 납을 대상으로 중금속의 세포내 흡수를 조사한 결과 16.59%와 35.38%의 중금속이 세포내로 흡수되었음을 확인하였다.

Keywords

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