KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Inhibitory Effect of Cacao Bean Husk Extract on Glucosyltransferase from Streptococus mutans B13

Cacao Bean Husk 추출물의 Glucosyltransferase 저해효과

  • 권익부 (롯데그룹중앙연구소 생물과학연구팀) ;
  • 이용우안봉전이신영 (롯데그룹중앙연구소 생물과학연구팀 롯데그룹중앙연구소 생물과학연구팀 강원대학교 발효공학과)
  • Published : 1993.03.01
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Abstract

The inhibitory effect of cacao bean husk (CBH) extract on glucosyltransferase(GTasc) from Streptococcus mutans B13 was investigated. Water solube extract from CBH showeda sarong inhibitory effect (88-89%) on GTase from Streptococcus mutans Bl3. GTase inhibitors from sequential extraction by hot water or water-methanol had the strongest inhibition. Sources, fermentation, and types of solvents and fumigation processes did not influence the effect. These active compounds proved to be polyphones through acid hydrolytic analysis of the precipitates by ammonium sulfate or ethanol and proteinase K. It was also confirmed by additional column chromatography of Sephadex G-50, Sephadex LH-20 and DEAE-Sephdex A-50.

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