Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of the S-type Pyocin of Pseudomonas aeruginwa 90-2-2205 Isolated from Korean Patients

한국환자유래의 녹농균 90-2-2205로부터 S형 Pyocin의 정제 및 특성

  • 김란숙 (경북대학교 자연과학대학 미생물학과) ;
  • 이정미 (경북대학교 자연과학대학 미생물학과) ;
  • 박영덕 (경북대학교 자연과학대학 미생물학과) ;
  • 진익렬 (경북대학교 자연과학대학 미생물학과) ;
  • 김병오 (경북대학교 자연과학대학 미생물학과)
  • Published : 1993.04.01
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Abstract

The s-type pyocin was purified from the lysate of the mitomycin C-induced Pseudomonas aeruginosa 90-2-2205 cells by the other of ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration. The purity was confirmed by the polyacry-lamide gel electrophoresis. The molecular weight of the purified pyocin was estimated 180, 000 by gel filtration. The pyocin was analyzed to be a complex of some polypeptides by the SDS-PAGE. The pyocin was stable by heat treatment and at pH 6-7.5 by adding 10-3% gelatin and 0.2M NaCl to the 10mM Tris-HCl buffer (pH7.5). Its killing action against the sensitive cells was assumably a single hit process.

Keywords

References

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