Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Tyrosinase-Inhibiting Isoflavonoids Produced by Streptomyces sp. 20747

Streptomyces sp. 20747이 생산하는 Tyrosinase-Inhibiting Isoflavonoids

  • 이충환 (KIST 유전공학연구소 미생물공학연구실) ;
  • 전효곤 (KIST 유전공학연구소 미생물공학연구실) ;
  • 고영희 (KIST 유전공학연구소 미생물공학연구실) ;
  • 서영배 (KIST 유전공학연구소 미생물공학연구실)
  • Published : 1993.04.01
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Abstract

Three isoflavonoids having tyrosinase-inhibiting activity were isolated from the culture filtrate of Streptomyces sp. 20747. Their structures were determined by UV, EI-MS, 1H-NMR, 13C-NMR to be daidzein, daidzein 7-rhamnoside, and genistein 7-rhamnoside, which were competitive with substrate and had IC50 value of 14, 19, and 16 ng/ml, respectively to mushroom tyrosinase and did not inhibit melanin production of Streptomyces bikiniensis. Soybean meal as well as peptone were found to be a good nitrogen source for tyrosinase-inhibiting isoflavonoids production, susgesting that soybean meal is not the origin of tyrosinase inhibiting isoflavonoids formation in Streptomyces sp. 20747 strain.

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References

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