Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Identification of Interleukin-l Like Material in Streptornyces Culture Supernatant

Streptomyces 배양액에서의 Interleukin-1 유사물질의 동정

  • Published : 1993.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

We have identified a T cell-activating material in the culture supernatant of Streptomyces species. The factor in microbial culture supernatant (MCS) induced thymocyte proliferation in a does dependent fashion and it could be detected by immunoblot analysis using anti-interleukin-1(IL-1) antibody. The factor in MCS was slightly larger(about 21 kd) in its molecular weight than IL-1 on SDS-PAGE. When 125I-MCS was covalently coupled with homo-bifunctional cross-linking agent, disuccinimidyl-propionate to IL-1 receptor(IL-1R) on mouse thymoma cell(EL-4) and immunoprecipitated with anti-IL-1R antibody the molecular weight of this complex of 110 kd was observed.

Keywords

References

  1. Actinomycetes in Biotechnology Goodfellow,M.;S.T.Williams;M.Mordarski
  2. TIBS v.17 Mammalian hormones in microbial cells Lenard,J.
  3. J. of Immunol. v.148 Cyclosporin A and FK506 mediate differential effects on T cell activation in vitro Bishop,D.K.;W.Li
  4. Science v.251 Chemistry and biology of the immunophilins and their immunosuppressive ligands Schreiber,S.L.
  5. Blood v.59 Cyclosporin A is selectively cytotoxic to human leukemic T cells in vitro Totterman,T.H.;A.Danersund;K.Niisson;A.Killander
  6. J. of Antibiot. v.39 Anovel method of screening for immunomodulating substances, extablishment of an assay system and its application to culture broths of microorganisms Nakamura,A.;K.Nagai;S.Suzuki;K.Ando;G.Tamura
  7. Nature v.352 Immunosuppressants at work DeFranco,A.L.
  8. J. Exp. Med. v.136 Potnetiation of the lymphocyte response to mitogens.Ⅰ. The responding cell Gery,I.;R.K.Gershon;B.H.Waksman
  9. Immunol. Today v.7 There is more than one interleukinⅠ Oppenheim,J.J.;E.J.Kovacs;K.Matsuchima;S.K.Durum
  10. Adv. Immunol. v.44 Interleukin-1 and its biologically related cytokines Dinarello,C.A.
  11. Proc. Natl. Acad. Sci. USA v.81 Nucleotide sequence of human monocyte interlueukin 1 precursor cDNA Auron,P.E.;A.C.Webb;L.J.Rosenwasser
  12. Nature v.315 Cloning, sequece and expression of two distint human interleukin 1 complementary DNAs March,C.J.;B.Mosley;A.Larsen
  13. Lymphokines and Interferons Clemens,M.J.;A.G.Morris;A.J.H.Gearing
  14. Kor. J. BRM. v.2 Identification and characterization of interleukin 1 inhibitor from human febrile urine Bae,Y.S.;E.H.Lee;D.Y.Yoon;M.S.Nam;K.H.Kim;I.S.Choe;T.W.Chung
  15. Anal. Biochem. v.112 Western blotting Burntte,W.H.