Abstract
The present study was undertaken to assess the role of cytokines in the activation of peritoneal macrophages from Toxoplasma-infected mice. Peritoneal macrophages from Toxoplasma-infected mice (10 cysts of Beverley strain/mouse) were harvested 8 weeks after infection, and incubated with the mitogen-induced lymphokine, recombinant mouse $interferon-{\gamma}(IFN-{\gamma})$, recombinant mouse tumor necrosis $factor-{\alpha}{\;}(TNF-{\alpha})$ alone or in combination with 4$IFN-{\gamma}(IFN-{\gamma}/TNF-{\alpha})$ for 24hr at 37^{\circ}C$, 5% $CO_2$. Macrophage activation was measured by the amount of $H_20_2{\;}and{\;}N0_2^{-}$ production, and antiToxoplasma activities of macrophages. $IFN-{\gamma}{\;}or{\;}IFN-{\gamma}/TNF-{\alpha}-treated$ macrophages from Toxoplasma-infected mice revealed significantly higher $H_20_2$ production than resident macrophages from Toxoplasma-infected mice. The production of $N0_2^{-}{\;}by{\;}TNF-{\alpha}-,{\;}IFN-{\gamma}-{\;}or{\;}IFN-{\gamma}/TNF-{\alpha}-treated$ macrophages from Toxoplasma-infected mice were significantly higher than that by resident macrophages, whereas lymphokine-treated group produced similar amount as that produced by resident macrophages. Anti-Toxoplasma activities of cytokinetreated macrophages from Toxoplasma-infected mice were Significantly higher than those of resident macrophages. $IFN-{\gamma}-treated$ macrophages were significantly increased production of $H_20_2{\;}and{\;}N0_2^{-}$, and anti-Toxoplasma activities of macrophages between normal and Toxoplasma-infected mice, whereas the other cytokine-treated groups were not significant differences between them. These data suggested that IFN-{\gamma}was the only one of cytokines capable of significantly activating the peritoneal macrophages from Toxoplasmainfected mice.
T. gonnii의 Beverley주를 감염시킨 마우스(감염군)로부터 분리한 복강대식세포에 cytokine의 종류 및 농도에 따른 대식세포의 활성화 정도를 평가하기 위하여 복강대식세포 단세포층에 medium, 조제 Iymphokine, 재조합 tumornecrosis $factor-{\alpha}{\;}(TNF-{\alpha})$. 재조합 $interferon-{\gamma}{\;}(IFN-{\gamma})$, 및 재조합 $IFN-{\gamma}와{\;}TNF-{\alpha}$를 함께($IFN-{\gamma}/TNF-{\alpha}$) 처치한 후, 각 처치군별 $H_2O_2{\;}생산량,{\;}NO2^{-}$ 생산량 및 T. gondii의 대식세포내 침투억제능을 측정하였다. 감염군의 복강대식세포에 $IFN-{\gamma}{\;}처치시{\;}NO2^{-}$ 생산량은 농도에 따라 유의하게 증가하였으나 그외의 처치군에서는 농도에 따른 유의한 차이가 없었다. 감염군 대식세포에 $IFN-{\gamma}나{\;}IFN-{\gamma}/TNF-{\alpha}$ 처치시 $H_2O_2$ 생산량이 medium처치군보다 유의하게 증가하였으며. $NO2^{-}$ 생산량은 $TNF-{\alpha},{\;}IFN-{\gamma}나{\;}IFN-{\gamma}/TNF-{\alpha}$ 처치시 유의하게 증가하였다. 감염군에 cytokine 처치시 T. gondii의 대식세포내 침투억제능은 medium 처치시보다 모두 증가되었다 또한 정상군과 감염군의 $H_2O_2$ 생산량, $NO2^{-}$ 생산량 및 T. gondii의 대식세포내 침투억제능을 상호 비교시 $IFN-{\gamma}$ 처치군은 유의한 차이를 나타냈으나 그 외의 cytokine 처치군에서는 유의한 차이를 나타내지 않았다. 이상의 성적으로 보아 $IFN-{\gamma}$가 Toxoplosma감염 마우스 복강대식세포의 활성화에도 중요한 역할을 함을 알 수 있었다.
