한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권3호
  • /
  • Pages.246-251
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Pseudomonas syringae pv. tabaci의 독소생성에 미치는 Phage의 영향

Influence of Phage on Production of Tabtoxin by Pseudomonas syringae pv.tabaci

  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 유진삼 (부산대학교 자연과학대학 미생물학과) ;
  • 성영림 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과)
  • 발행 : 1994.06.01
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초록

Pseudomonas syringae pv. tabaci(Pa45) Tox$^{-}$ cells were infected with phage Ps90 strain isolated form the natural source, and the Ps90 lysogenized bacterial cells were then obtained. The lyxohenized cells produced tabtoxin and the phage induction occured when the cells treated with mitomycin C. The Southren hybridization alnalysis of the four EcoRI-treated plasmid fragments and the EcoRI-digested genomic DNA of Tox$^{+}$ and Tox$^{-}$ strains using phage DNA as a probe showed that only those DNA fragment of Tox$^{+}$ strain were related to the Ps90 phage DNA. Based on these results, the tabtoxin producing DNA fragments of the bacteris are presumed to have originated from the same phage DNA, and to be responsible for the pathogenecity of the bactrial strains.

키워드

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