Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High Cell Density Culture of Alcaligenes eutrophus and Poly-$\beta$-hydroxybutyrate Production by Optimization of Medium Compositions

배지조성 최적화를 통한 Alcaligenes eutrophus의 고농동 세포배양 및 Poly$\beta$-hydroxybutyrate 생산

  • 이용우 (서울대학교 공과대학 화학공학과) ;
  • 유영제 (서울대학교 공과대학 화학공학과)
  • Published : 1994.08.01
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Abstract

The medium compositions of Alcaligenes eutrophus were optimized for increasing PHB productivity. It is very important to optimize the concentrations of inorganic salts and trace eleme- nts as well as carbon and nitrogen sources to maximize cell growth rate and productivity. The fed-batch culture of Alcaligenes eutrophus by dual feeding of ammonia water and glucose under optimized initial medium concentrations was carried out. Glucose was fed manually according to glucose consumption rate and ammonia water by pH-stat. The final cell concentrations and PHB content in 30 hours were 122 g/l and 65% of dry cell weight(yielding 79 g of PHB/l), respectively and 2.64 g/l/hr of PHB production rate was obtained.

Keywords

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