한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권6호
  • /
  • Pages.665-671
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  • 1994
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Erwinia sp.에 의한 Gallic Acid로부터 Pyrogallol의 생산

Production of Pyrogallol from Gallic Acid by Erwinia sp.

  • 발행 : 1994.12.01
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초록

For the production of pyrogallot from gallic acid, about 100 strains of bacteria capable of assimilating gallic acid as a sole carbon and energy source were isolated from the soil. JH- 004 strain showing the highest activity of gallate decarboxy#lase was selected from them and identi- fied as Erwinia sp. The optimal conditions for the production of pyrogallol from gallic acid were examined. The resting cells of JH-004 cultured in a complex medium containing 0.2%(w/v) gallic acid were prepared after the treatment of the pellet with a freezing and thawing, and used as a enzyme source. The reaction mixtures for the maximal production of pyrogallol were shown to be 6 g/l of resting cells and 15 g/l of gallic acid in 25 mM potassium phosphate buffer. The optimal pH for the reaction was 5.0 and the optimal temperature was 35$\circ$C . Additionally, Triton X-100(0.01%, w/v) was found to be most effective for the production of pyrogallol. Under the above conditions, 10.27 g/l of pyrogallol was produced from 15 g/l of gallic acid after incubation of 35 hrs. This amount of pyrogallol corresponds to a 92.37% yields, based on gallic acid.

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