Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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A Study on the Racemization of Amino acids and its Separation with GC, GC/MS and HPLC

아미노산의 광학이성화 및 GC, GC/MS, HPLC에 의한 광학이성질체의 분리에 관한 연구

  • Rhee, Jae-Seong (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Hong, Jong-Ki (Basic Science Supporting Center, Korea Research Institute of Standards and Science) ;
  • Eo, Yun-Woo (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Kim, Taek-Jae (Department of Chemistry, Kyung Ki University)
  • 이재성 (한국과학기술연구원 특성분석센터) ;
  • 홍종기 (대덕 표준과학연구원 기초과학지원센터) ;
  • 어연우 (한국과학기술연구원 특성분석센터) ;
  • 김택제 (경기대학교 화학과)
  • Received : 1994.01.21
  • Published : 1994.03.25
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Abstract

The importance of separation comes from demands on study for exact effect of synthetic drugs and the reactivity of enantiomer in biological system. Racemization rate was measured under the influence of heat, acid, UV-light, enzyme(trypsin) and 6N-HCl at $105^{\circ}C$ on alanine, threonine, isoleucine, lecuine, aspartic acid, methionine, glutamic acid, tyrosine. The method for the identification of overlapped amino acids with GC was developed from the close study of fragmentation pattern with mass spectrometry. With cyclodextrin bonded phase by HPLC, the separation of dansyl amino acid was tested for compartison.

아미노산 중 alanine, threonine, isoleucine, leucine, aspartic acid, methionine, glutamic acid, tyrosine을 대상으로 열처리, 산처리, UV 광선 조사, 효소(trypsin)의 영향을 살펴보고 6N-HCl, $105^{\circ}C$로 가열했을 때 이성화하는 정도를 측정하였다. 또한 Mass Spectrometer를 이용할 때 각 아미노산의 fragment되는 과정을 조사하여 GC에서 서로 겹칠 때 분석하는 법을 개발하였다. HPLC에서 cyclodextrin bonded phase를 이용해서 dansyl amino acid의 분리를 시도하였다.

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