Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Extraction and Characterization of Antibacterial Components from the Roots of Evening Primrose (Oenothera odorata Jacquin)

달맞이꽃(Oenothera odorata Jacquin) 뿌리에서 추훌한 황균성분의 특성

  • Sin, Sung-Jin (Yusung Agricultural High School) ;
  • Kwon, Soon-Kyung (Dept. of Agricultural Chemistry, College of Agriculture, Chungnam National University) ;
  • Lee, Kweon-Haeng (Biotechnology Lab., R & D Center, Samho Co., Ltd.) ;
  • Sung, Nack-Do (Dept. of Agricultural Chemistry, College of Agriculture, Chungnam National University) ;
  • Choi, Woo-Young (Dept. of Agricultural Chemistry, College of Agriculture, Chungnam National University)
  • 신성진 (유성농업고등학교) ;
  • 권순경 (충남대학교 농과대학 농화학과) ;
  • 이권행 (삼호울산(주) 종합연구소) ;
  • 성낙도 (충남대학교 농과대학 농화학과) ;
  • 최우영 (충남대학교 농과대학 농화학과)
  • Published : 1994.06.30
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Abstract

An antibacterial component was isolated from the roots of evening primrose(Oenothera odorata Jacquin). The component was purified by repeated preparative TLC of the chloroform-extract with solvent system of toluene(7):ethyl acetate(4):formic acid(O.8). NMR spectrum indicated that it is a steroid compound containing radical(-C=C-C=0) and aromatic structures. Its antibacterial activity against strains of the genus Streptococcus was relatively high, the MIC value was 50 acccording to the Hoechst Manual-345, as compared with the other genera such as Staphylococcus and Escherichia.

달맞이꽃 (Oenothera odorata Jacquin)의 뿌리로 부터 천연항균성분을 분리하고 그 특성을 조사하였다. 주성분은 chloroform 추출분획중에 존재하는 TLC의 Rf값 0.39[전개용매계, toluene : ethyl acetate : formic acid (7 : 4 : 0.8)]의 것이였으며 NMR 분석결과 방향족고리를 포함하는 steroid계통의 화합물로서 장파장의 자외선(365nm)을 흡수하며, Streptococcus속 균주에 대한 항균활성(MIC: 50)이 Staphylococcus 및 Escherichia속 균주에 대한 활성(MIC: 100~1600) 보다 월등히 높았다.

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