Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of $\beta$-1,4-D-arabinogalactanase Producing Strain and Enzyme Purification

$\beta$-1,4-D-arabinogalactanase 생산균주의 분리 및 효소정제

  • 신해헌 (연세대학교 식품-생물공학과 및 생물산업소재연구센터) ;
  • 변유량 (연세대학교 식품-생물공학과 및 생물산업소재연구센터)
  • Published : 1995.12.01
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Abstract

Alkalophilic Bacillus sp. HJ-12 producing $\beta $-1, 4-D-arabinogalactanase was isolated from soil in the alkalic condition, pH 10.0. $\beta $-1, 4-D-arabinogalactanase was maximaly produced in the medium consisting of 2% soybean arabinogalactan (SAG), 0.5% yeast extract, 0.5% polypeptone, 0.5% NaCl, 0.1% K$_{2}$HPO$_{4}$, 0.02% MgSO$_{4}$$\cdot $7H$_{2}$O, 0.1% Na$_{2}$CO$_{3}$ under the aerobic condition (pH 8.2). $\beta $-1, 4-D-arabinogalactanase is inducible enzyme so that its activity has been increased 10 fold in the SAG medium than in the glucose medium. Through the ammonium sulfate precipitation, DEAE- Sephadex A-50 ion chromatography, and Sephadex G-75 gel chromatography procedures, this enzyme was purified with a single protein of 11% vield and 110 fold's purity. $\beta $-1, 4-D-arabinogalactanase is endo type enzyme producing ollgosaccharide from SAG.

Keywords

References

  1. Cereal Foods World v.34 Nutritional and physical properties of dietary fiber from soybeans Lo,G.S.
  2. Phytochemistry v.18 Form and function of arabinogalactans and arabinogalactan-proteins Clarke,A.E.;R.L.Anderson;B.A.Stone
  3. J. Biol. Chem. v.251 Structure of plant cell walls Labavitch,J.M.;L.E.Freeman;P.Albersheim
  4. Adv. Carbohydr. Biochem. v.32 Hemicelluases: their occurrence, purifiation, properties and mode of action Dekker,B.F.H.;G.N.Richards
  5. L. seeds. Planta v.192 Purification and properties of β-galactosidase or exo(1->4)-β-D-galactanase from the cotyledons of germinated Lupinus angustifolius Buckeridge,M.S.;J.S.Grant Reid
  6. 日本食品工業學會誌 v.19 大豆炭水化物の醱酵分解に關する硏究 木內 幹;太田輝夫;藤家宖子;海老根英雉
  7. Agr. Biol. Chem. v.36 Purification and properties of several galactanase of Bacillus subtilis var amylosacchariticus Emi,S.;T.Yamamoto
  8. Eur. J. Biochem. v.193 Purification and characterization of exo-1,4-β-galactanase from a strain of Bacillus subtilis Nakano,H.;S.Takenishi;S.Kitahara;H.Kinugasa;Y.Watanabe
  9. Agr. Biol. Chem. v.55 Purification and properties of two galactanase from Penicillium citrinum Tsumura,K.;T.Hashimoto;T.Akida;K.Horikoshi
  10. Agr. Biol. Chem. v.49 Purifacation and properties of two galactanase from Penicillium citrinum Nakano,H.;S.Takenishi;Y.Watanabe
  11. Carbohydr. Biochem. v.15 Endo-β-1,4-D-galactanase from Aspergillus niger var. aculeatus: Purification and some properties Lahaye,M.;J.Vigouroux;J.F.Thibault
  12. 한국식품과학회 심포지움 올리고당의 기능성 박관화;정기섭;H.Hidaka;S.Kitahata;서진호;Y.Kaji
  13. Bergey's Manual of Determinative Bacteriology(7th ed.)
  14. J. Biol. Chem. v.195 Notes on sugar determination Somogyi,N.
  15. J. Biol. Chem. v.153 A photometric adaption of the somogyi method for the determination of glucose Nelson,N.
  16. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois,M.;K.A.Gills;J.K.Hamilton;P.A.Rebers;F.Smith
  17. Agr. Biol. Chem. v.29 Polysaccharides of soybean seeds. Part Ⅰ. Polysaccharide constituents of hot-water-extract fraction of soybean seeds and an arabinogalactan as its major component Morita,M
  18. Nature(London) v.227 Changes of structural proteins during the assembly of the head of bacteriphage T4 Laemnli,U.K.
  19. Anal. Biochem. v.72 Bradford,M.
  20. Alkalophilic Microorganisms: A new microbial world. Horikoshi,K.;T.Akiba