Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Control of Organic Acid Inhibition in Escherichia coli Culture with Eledtroudialysis

유기산의 저해를 조절하기 위한 Escherichia coli의 전기투석배양

  • Published : 1995.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

E. coli growth is inhibited by organic acids produced in the broth. In order to reduce the inhibition, an electrodialysis unit was used. Model solutions (acetic acid plus distilled water or M-9 medium) were tested in the unit for investigating the optimum condition of current and voltage. Electrodialysis cultures were performed with the optimum condition where the highest current efficiency could be attained. The distilled water plus acetic acid gave us a higher current efficiency than the M-9 plus acetic acid. Electrodialysis efficiently removed acetic acid and so enhanced the specific growth rate of E. coli compared with the control experiment without clectrodialysis.

Keywords

References

  1. Ph. D. Thesis. Dept. of Chem. Eng. Univ. Cal. A study of acetic acid formation in Escherichia coli fermentation Han,K.H.
  2. Bacteriol. Rev. v.33 Dialysis culture of microorganisms: design;theory,and results Schultz,J.S.;P.Gerhardt.
  3. Adv. Biochem. Eng. v.24 Purification of enzymes by liquid-liquid extraction Kula,M.R.;K.H.Kroner;H.Hustedt
  4. Biotechnol. Bioeng. v.26 Economic evaluation of alternative ethanol fermentation processes Maiorella,B.L.;H.W.Blanch;C.R.Wilke
  5. J. Bacteriol. v.86 Dialysis flask for concentrated culture of microorganisms Gerhardt,P.;D.M.Gallup
  6. J. Appl. Environ. Microbiol. v.54 Acetic acid production by an elelctrodialysis fermentation method with a computerized control system Nomura,Y.;M.Iwahara;M.Hongo
  7. Biotechnol. Prog. v.8 Recovery of propionic and acetic acid from fermentation broth by electrodialysis Weier,A.J.;B.A.Glatz;C.E.Glatz
  8. J. Ferm. Bioeng. v.75 no.4 Production and recovery of propionic and acetic acid in electrodialysis culture of Propionibacterium shermanii Zhang,S.T.;H.Matsuoka;K.Toda
  9. J. Ferm. Bioeng. v.77 no.3 Kinetic study of celectrodialysis of acetic acid and phosphate in fermentation broth Zhang,S.T.;K.Toda
  10. 한국생물공학회지 v.9 no.5 대장균의 실관투석배양 김인호;윤태호
  11. Desalination v.7 Behavior of an electrodialsis unit cell Mas,L.J.;P.M.Pierrard;P.A.Prax;J.C.Sohm
  12. J. Bacteriol. v.144 Are growth rates of Escherichia coli in batch cultures limited by respiratio? Anderson,K.B.;K.Meyenburg