Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Acinetobacter sp. BE-254에 의한 유화제의 생산

  • 김순한 (부산대학교 미생물학과) ;
  • 임이종 (부산대학교 미생물학과) ;
  • 최경숙 (부산대학교 미생물학과) ;
  • 정영기 (동의대학교 미생물학과) ;
  • 장경립 (부산대학교 미생물학과) ;
  • 이태호 (부산대학교 미생물학과)
  • Published : 1996.04.01
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Abstract

The strain producing bioemulsifier was isolated from soil samples. The isolated strain was identified as the genus Acinetobacter through its morphological, cultural and physiological characteristics. The highest emulsification activity and stability by Acinetobacter sp. BE-254 was observed after 5 days of cultivation in the culture medium containing n-hexadecane 4%, NaNO$_{3}$ 0.2%, KH$_{2}$PO$_{4}$ 0.01%, MgSO$_{4}$-7H$_{2}$O 0.01%, CaCl$_{2}$ 0.01%, and yeast extract 0.01%. The optimum pH and temperature for bioemulsifier production were pH 7.0 and 30$\circ$C, respectively. Furthermore the most of bioemulsifier was produced during the exponential growth phase, and this suggested that the bioemulsifier production was growth-associated. The bioemulsifier showed good emulsification activity on various emulsifying substrates such as hydrocarbons, edible oils, and petroleum fractions.

Keywords

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