Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High Cell Density Cultivation of Pseudomonas putida BM01 Using Glucose

  • Kim, Guk Jin (Bioprocess Technology Research Group, Korea Research Institute of Bioscience and Biotechnoloug) ;
  • In Young Lee (Bioprocess Technology Research Group, Korea Research Institute of Bioscience and Biotechnoloug) ;
  • Dae Keon Choi (Bioprocess Technology Research Group, Korea Research Institute of Bioscience and Biotechnoloug) ;
  • Sung Chul Yoon (Department of Microbiology, Geongsang national University) ;
  • Young Hoon Park (Bioprocess Technology Research Group, Korea Research Institute of Bioscience and Biotechnoloug)
  • Published : 1996.06.01
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Abstract

Pseudomonas putida BM01 was grown efficiently on glucose as the sole carbon source with a supply of a nitrogen source in pH-stat mode using a low setpoint limit. A final cell concentration of 100 g/l was obtained in 30 h of fed-batch cultivation by controlling glucose concentration within the range of 5-20 g/l and maintaining dissolved oxygen tension above 10$%$ saturation using pure oxygen. This high cell density culture technique is believed highly useful for the production of poly(3-hydroxyalkanoates) by this strain.

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