중금속이 봄여뀌(Persicaria vulgaris Webb. et Moq.)의 항산화효소활성에 미치는 영향

Effect of Metals on Anti - Oxidase Activity in Persicaria vulgaris Webb. et Moq.

중금속(Cd, Cu, Zn, Al) 처리하에서 생육시켰을때 봄여뀌(persicaria vulgaris Webb. et Moq)의 부위별 항산화효소활성 및 동위효소 pattern을 조사해 본 결과는 다음과 같다. 1. 잎과 줄기의 SOD활성은 감소되었으나, 뿌리에서는 증가되었다. 특히 Al처리하에서 자란 뿌리는 높은 SOD활성을 나타내었다. 2. Cd, Cu처리하에서 POD 활성은 중금속 및 농도간에 일정한 경향을 나타내지 않았고, Zn, Al 처리하에서는 억제되었으나, Zn 5000ppm, Al 500ppm 하에서 생육시킨 뿌리에서는 높은 활성을 나타내었다. 3 중금속처리시 CAT 활성은 잎에서 감소되었고, 뿌리에서는 농도가 증가할수록 활성이 뚜렷이 높아졌다. 특히, Al처리하에서 자란 뿌리가 가장 높은 활성을 나타내었다. 4 중금속처리에 따른 동위효소 pattern은 POD의 경우 한 개 band에서 동위효소의 출현없이 강도의 크기 변화만 나타났고, SOD의 동위효소 pattern 변화는 다양하였다.

To study the effects of metal ions on the activities of antioxidative enzymes, the activities of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) of Persicaria vulgaris has been studied after treating with Cd, Cu, Zn and Al. 1. The activities of SOD in leaf and stem were decreased, but that in root was increased. Among the metal ions studied in this report, Al gave the highest increase in SOD activity in root. 2. The activities of POD after treating with Cd or Cu did not show any significant differences. POD activities after treating with Zn and Al has been decreased, however, that in root showed increased activities after treating with Zn 5,000 ppm or Al 500 ppm. 3. The activity of CAT in leaf was decreased with every metals studied. The CAT activity in root was increased with increased concentration. The root treated with Al showed highest activity. 4. The presence of isozymes after treated metal ions has been studied in gel electrophoresis. The POD treated plant did not show any new isozymes, but the intensity of one of pre-existent band was increased. The SOD treated plant showed the several new isozymes.