The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Ijingtang fractions on activities of NaK ATPase and Ca ATPase in rat brain synaptosome

이진탕 분획엑스가 synaptosomal NaK ATPase와 Ca ATPase의 활성도에 미치는 영향

  • 조영욱 (경희대학교 의과대학 생리학교실) ;
  • 한승호 (경희대학교 의과대학 생리학교실) ;
  • 김창주 (경희대학교 의과대학 생리학교실) ;
  • 민병일 (경희대학교 의과대학 생리학교실) ;
  • 이태희 (경원대학교 한의과대학 본초학교실) ;
  • 윤상협 (경희대학교 동서의학연구소) ;
  • 오수명 (경희대학교 동서의학연구소)
  • Published : 1997.04.30
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Abstract

To explore the action mechanism of Ijintang in the brain, the authors investigated the effects of Ijintang fractions on MgNaK ATPase and MgCa ATPase in rat brain synaptosomes prepared from cerebral cortex. The activities of MgNaK ATPase and MgCa ATPase were assayed by the level of inorganic phosphate liberated from the hydrolysis of ATP. Fraction WH-95-7 at the concentration of $10^{-2}%$ decreased the activity of MgNaK ATPase about 34.1% and also reduced the activity of MgCa ATPase about 49.3% But, other fractions (WB-95-7, WC-95-7, MB-95-7, MC-95-7, MH-95-7) did not significantly changed the activities of the MgNaK ATPase and MgCa ATPase The decreased activity of MgNaK ATPase by WH-95-7 will decrease the rate of $Ca^{2+}$ efflux, probably via an Na-Ca exchange mechanism and will increase the rate of $Ca^{2+}$ entry by the depolarization of nerve terminals. The reduced activity of MgCa ATPase by WH-95-7 will result in the decreased efflux of $Ca^{2+}$. As a conclusion, it can be speculated that lithium elevates the intrasynaptosomal $Ca^{2+}$ concentration via inhibition of the activities of MgNaK ATPase and MgCa ATPase. and this increased $[Ca^{2+}]i$ will cause the release of neurotransmitters.

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