Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Growth and Differentation of Rat Mammary Epithelial Cells Cultured in Serum-free Medium

  • Kim, Dong-Yeum (College of Pharmacy, and Research Institute of Drug Development, Pusan National University) ;
  • Jhun, Byung-Hak (College of Pharmacy, and Research Institute of Drug Development, Pusan National University) ;
  • Lee, Kyung-Hee (College of Pharmacy, and Research Institute of Drug Development, Pusan National University) ;
  • Hong, Seung-Chul (College of Pharmacy, and Research Institute of Drug Development, Pusan National University) ;
  • Clifton, Kelly-H. (Department of Human Oncology, K4/330 CSC, University of Wisconsin Comprehensive Cancer Center) ;
  • Kim, Nam-Deuk (College of Pharmacy, and Research Institute of Drug Development, Pusan National University)
  • Published : 1997.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies developed within a week and remained viable for months in culture. Upon subculture of one-week-old primary colonies, almost the same morphology of colonies was developed. The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with prolactin, $E_2$, and progesterone (complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By flow cytometry with FITCPNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. It is likely that combined prolactin, cortisol, and insulin in CHSFM stimulate terminal differentiation of clonogenic cells.

Keywords

References

  1. Biochem. Biophys. Res. Commun. v.120 Elongated cells derived from rat mammary cuboidal epithelial cell lines resemble cultured mesenchymal cells in their pattern of protein synthesis Barraclough,R.;Dawson,K.J.;Rudland,P.S.
  2. J. Cell. Physiol. v.131 Differential control of mRNA levels for Thy-1 antugen and laminin in rat mammary epithelial and myoepithelial-like cells Barraclough,R.;Kimbell,R.;Rudland,P.S.
  3. Cancer Res. v.47 Contact insensitivity of a subpopulation of normal human fetal kidney epithelial cells and of human carcinoma cell lines Chang,C.C.;Trosko,J.E.;El Fouly,M.H.;Gibson D'Ambrosio,R.E.;D'Ambrosio,S.M.
  4. J. Natl. Cancer Inst. v.55 Mammary carcinogenesis-enhancing effect of adrenalectomy in irradiated rats with pituitary tumor MtT-F4 Clifton,K.H.;Sridharan,B.N.;Douple,E.B.
  5. Cancer Res. v.38 Effect of radiation type and role of glucocorticoids, gonadectomy and thyroidectomy in mammary tumor induction in MtT-grafted rats Clifton,K.H.;Crowley,J.
  6. Cell Clones: Manual of Mammalian Cell Techniques Clonogen transplantation assay of mammary and thyroid epithelial cells Clifton,K.H.;Gould,M.N.;Potten,C.S.(ed.);Hendry,J.H.(ed.)
  7. Scientific Issues in Quantitative Cancer Risk Assessment The clonogenic cells of the rat mammary and thyroid glands: Their biology, frequency of initiation and promotion/progression cancer Clifton,K.H.;Moolgavkar,S.H.(ed.)
  8. Dev. Biol. v.127 Ductal morphogenesis in the mouse mammary gland: evidence supporting a role for epidermal growth factor Coleman,S.;Silberstein,G.B.;Daniel,C.W.
  9. Exp. Cell Res. v.174 Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1 Deeks,S.;Richards,J.;Nandi,S.
  10. Cancer Res. v.46 Marker evolution during the development of the rat mammary gland: stem cells identified by markers and the role of myoepithelial cells Dulbecco,R.;Allen,W.R.;Bologna,M.;Bowman,M.
  11. J. Cell. Physiol. v.132 Influence of hormone and growth factor interactions on the proliferative potential of normal rat mammary epithelial cells Ethier,S.P.;Kudla,A.;Cundiff,K.C.
  12. Exp. Cell Res. v.107 Morphological and quantitative studies of gland formation from inocula of monodispersed rat mammary cells Gould,M.N.;Biel,W.F.;Clifton,K.H
  13. Vitro v.26 Primary culture of normal rat mammary epithelial cells within a basement membrane matrix I. Regulation of proliferation by hormones and growth factors Hahm,H.A.;Ip,M.M.
  14. Proc. Natl. Acad. Sci. USA v.81 Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract Hammond,S.L.;Ham,R.G.;Stampfer,M.R.
  15. Endocrinology v.116 Stimulation of mammary epithelial cell growth in vitro: interaction of epidermal growth factor and mammogenic hormones Imagawa,W.;Tomooka,Y.;Hamamoto,S.
  16. Int. J. Radiat. Biol. v.59 Repair of potentially lethal damage in rat mammary clonogens following irradiation in organoid culture Kamiya,K.;Kum,N.D.;Gould,M.N.;Clifton,K.H.
  17. Growth of cells in hormonally defined medium v.2 Effects of growth factors on mammary epithelial cell proliferation and basement membrane synthesis Kidwell,W.R.;Salomom,D.S.;Liotta,L.A.;Barnes,D.(ed.);Sato,G.(ed.)
  18. Exp. Cell Res. v.207 Characterization of rat mammary epithelial cell subpopulations by peanut lectin and anti-Thy-1.1 antibody and study of flow sorted cells in vivo Kim,N.D.;Clifton,K.H.
  19. Matrigel. Exp. Cell Res. v.209 Primary culture of flow cytometry-sorted rat mammary epithelial cell (RMEC) subpopulations in a reconstituted basement membrane Kim,N.D.;Oberley,T.D.;Clifton,K.H.
  20. J. Korean Soc. Food Nutr. v.24 Study of rat mammary epithelial stem cells in vivo and in vitro Kim,N.D.;Piak,K.J.
  21. J. Cancer Res. Clin. Oncol. v.93 The presence and significance of the Thomsem-Friedenreich antigen in mammary gland Klein,P.J.;Newman,R.A.;Mller,P.;Uhlenbruck,G.;Citoler,P.;Schaefer,H.E.;Lennartz,K.J.;Fischer,R.
  22. J. Cell. Physiol. v.125 Differential reponse of normal rat mammary epithelial cells to mammogenic hormones and EGF McGrath,M.;Palmer,S.;Nandi,S.
  23. Cell Tissue Res. v.206 Association of Thy-1 cell surface differentiation antigen with certain connective tissues in vivo Morris,R.J.;Ritter,M.A.
  24. J. Natl. Cancer Inst. v.63 Binding of peanut lectin to breast epithelium, human carcinomas and a cultured rat mammary stem cell and its use as a marker of mammary differentiation Newman,R.A.;Klein,P.J.;Rudland,P.S.
  25. J. Cancer Res. Clin. Oncol. v.93 The presence and significance of the Thomsen-Friedenreich antigen in breast cancer.Ⅰ. Serological studies Newman,R.A.Klein,P.J.;Uhlenbruck,G.;Citoler,P.;Karduck,D.
  26. Br. J. Cancer v.27 The transplantation Kinetics of tumour cells Porter,E.H.;Hewitt,H.B.;Blake,E.R.
  27. J. Cell Sci. v.10 no.SUP Stem cells in mammary gland differentiation and cancer Rudland,P.S.;Barraclough,R.
  28. J. Histochem. Cytochem. v.39 Generation of lobuloalveolar development from isolated rat mammary ducts and end buds Rudland,P.S.
  29. Proc. Natl. Acad. Sci USA v.78 Differential response to growth factgor by rat mammary epithelium plated on different collagen substrata in serum-free medium Salomon,D.S.;Liotta,L.A.;Kidwell,W.R.
  30. Toxicol. Lett. v.49 Stem cell theory of carcinogenesis Trosko,J.E.;Chang,C.C.
  31. J. Cell. Physiol. v.132 Local effects of EGF, TGF-, and EGF-like growth factors on lobuloalveolar development of the mouse mammary gland in vivo Vonderhaar,B.K.
  32. J. Cell Sci. v.79 Characterization of rat mammary cell types in primary culture: lectins and antisera to basement membrane and intermediate filament proteins as indicators of cellular heterogeneity Warburton,M.J.;Ferns,S.A.;Hughes,C.M.;Rudland,P.S.
  33. Proc. Natl. Acad. Sci USA v.82 Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor Welte,K.;Platzer,E.;Lu,L.;Gabrilove,J.L.;Levi,E.;Mertelsmann,R.;Moore,M.A.
  34. Cell Biol. Int. Rep. v.6 Serum-free primary culture of human normal mammary epithelial cells in collagen gel matrix Yang,J.;Larson,L.;Flynn,D.