BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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5' Processing of RNA I in an Escherichia coli Strain Carrying the rnpA49 Mutation

  • Jung, Young-Hwan (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Park, Jung-Won (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Kim, Se-Mi (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Cho, Bong-Rae (Department of Chemistry, Chongju University) ;
  • Lee, Young-Hoon (Department of Chemistry, Korea Advanced Institute of Science and Technology)
  • Received : 1997.02.28
  • Published : 1997.03.31
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Abstract

RNA I. a negative controller of ColE1-type plasmid replication, is metabolized by several RNases in Escherichia coli. Two small derivatives of RNA I are accumulated at nonpermissive temperatures in an E. coli strain carrying the rnpA49 mutation, a thermosensitive mutation in the rnpA gene encoding the protein component of RNase P. A primer extension analysis was carried out to compare 5' processing of RNA I in the E. coli rnpA49 cells at both permissive and nonpermissive temperatures. Derivatives of RNA I having different 5' ends were observed in the cells grown at permissive and nonpermissive temperatures. Some of the derivatives may be generated by the cleavage of RNase P.

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