Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Isolation and Identification of Chondroitin Sulfates from the Mud Snail

  • Lee, Kyung-Bok (Department of Chemistry, Konyang University) ;
  • Kim, Jong-Sig (Department of Chemistry, Konyang University) ;
  • Kwak, Sang-Tae (Department of Biochemistry, School of Medicine, Konyang University) ;
  • Sim, Won-Bo (Department of Chemistry, Konyang University) ;
  • Kwak, Jong-Hwan (division of Applied Science, Korea Institute of Science and Technology) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, Seoul National University)
  • Published : 1998.10.01
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Abstract

chondroitin sulfates were isolated from the mud snail. For the quantitative analysis of enzymatic digestion products of isolated chondroitin sulfates, strong anion exchange-high performance liquid chromatography (SAX-HPLC) was performed. by the action of chondroitinase ABC, three unsaturated disaccharides$ 2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-D-galactose $$({\Delta}Di-OS), $2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose ({\Delta}Di-6S) and 2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose ({\Delta}Di-4S)$ were produced from the mud snail chondroitin sulfates. The analysis showed that relative proportion of ${\Delta}Di-OS/{\Delta}Di-6S/{\Delta}Di-4S$ was 58.7/3.1/38.2. The immunomodulating activity of chondroitin sulfate was examined by cell proliferation assay and these results suggest that it might be a immunosuppressant.

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References

  1. J. Biol. Chem. v.271 A new glycoaminoglycan from the giant african snail Achatina fulica Kim;Y.S.;Jo;Y.Y.;Chang;I.M.;Toida;T.;Park;Y.;Linhardt;R.J.
  2. Anal. Biochem. v.93 A partial modification of the carbozole method of Bitter and Muir for quanrification of hexauronic acids Koaki;M.;Yosizawa;Z.A.
  3. Biochem. J. Mappoing and quantification of the major oligasaccharides components of heparin Linhardt;R.J.;Rice;K.G.;Kim;Y.S.;Lohse;D.L.;Wzng;H.M.;Loganathan;L.
  4. J. Biol. Chem. v.259 Isolation and structural studies of heparen sulface and chondroitin sulfates from three species of molluscs Nader;H.B.;Ferreira;T.M.P.C.;Paiva;J.F.;Medeiros'M.G.L.;Jeronimo;S.M.B.;Paiva;V.M.P.;Dietrich;C.P.
  5. J. Exp. Med. v.182 Chondroitin sulfate A is a cell surface receptor for Plasmodium falcilarum infected erythrocytes Rogerson;S.J.;Chaiyaroj;S.C.;Reeder;J.C.;Brown;G.V.
  6. J. Biol. Chem. v.264 Proteoglycans in cell regulation Rooslahti;E.
  7. J. Bio. Chem. v.256 Changes of sulfated mucopolysaccharides and mucopolysaccharidase during fetal development Sampaio;J.O.;Dietrich;C.P.
  8. Biochem. Biophys. Acta. v.1241 Intracellular membranes in the synthesis;transport;and metabolism of proteoglycans Silbert;J.E.;Sugumaran;G.