KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Purification and Characteristics of New Biopolymer Produced by Alkaline-Tolerant Bacillus sp.

알칼리 내성 Bacillus sp.가 생산하는 생물 고분자의 정제 및 특성

  • Lee, Shin-Young (Division of Environmental and Biological Engineering, Kangwon National University) ;
  • Won, Suk (Division of Environmental and Biological Engineering, Kangwon National University) ;
  • Kang, Tae-Su (Kangwon provincial RDA) ;
  • Lee, Myong-Yurl (Department of Food Science and Technology, Chungju National University) ;
  • Lew, In-Deok (Department of Food Science and Technology, Chungju National University) ;
  • Kim, Jin-Young (Kim Jung Moon Aloe Co, Ltd)
  • 이신영 (강원대학교 환경.생물공학부) ;
  • 원숙 (강원대학교 환경.생물공학부) ;
  • 강태수 (강원도 농촌진흥원) ;
  • 이명열 (충주산업대학교 식품공학과) ;
  • 류인덕 (충주산업대학교 식품공학과) ;
  • 김진영 ((주) 김정문 알로에 연구소)
  • Published : 1998.10.01
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Abstract

Biopolymer from alkaline-tolerant Bacillus so. was purified, and its physico-chemical and structural properties were investigated. Crude biopolymer, precipitated by acetone from culture broth was fractionated into two fractions by gel chromatography on Sephadex G-200. Among two fractions, one fraction(PS I), which an acidic biopolymer precipitated by the CPC(cetylpyridinium chloride) treatment was studied further. PS I fraction had carboxyl groups and was positive at color reaction of sugar. PS I fraction also showed UV absorbance at 190-225nm. The purified acidic biopolymer was composed of 4% glucose, 8% glucosamine and 88% glutamic acid. Sugar components of the purified acidic biopolymer seemed to be linked to PGA(polyglutamic acid) which existed in the from of ${\gamma}$-peptide bond. By the results of Smith degradation of sugar components, glucose and glucosamine was bound by 1,3 glocosidic linkage. Therefore, this biopolymer was a glycopeptide, oligosaccaride ${\gamma}$-PGA. We concluded that the equivalent weight and the molecular weight of this biopolymer were estimated as about 171 and 5x105 dalton, respectively.

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