Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Transcriptional Induction of a Carbon Starvation Gene during Other Starvation and Stress Challenges in Pseudomonas putida MK1: A Role of a Carbon Starvation Gene in General Starvation and Stress Responses

  • Chitra, Subramanian (Environmental Biology, Division of Life Science, Catholic University of Korea) ;
  • Lee, Ho-Sa (Department of Biology, Kyunghee University) ;
  • Kim, Youngjun (Environmental Biology, Division of Life Science, Catholic University of Korea)
  • Published : 1999.09.01
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Abstract

Thirteen transcriptionally-fused carbon starvation mutants, derived from Pseudomonas putida ATCC 12633, were analyzed for their survivability and transcriptional induction profiles upon carbon starvation. One of these mutants, MK114, which exhibited the lowest survivability and the highest induction rate, was selected and further examined under different starvation (nitrogen and phosphate) and stress (osmolarity, H2O2, salts, alcohol, and heat) conditions. Under all tested conditions MK114 induced ${\beta}$-galactosidase activity, implying that the interrupted gene (cst114) is a general starvation and stress response gene. The rate of induction ranged from 2.6-fold for phosphate starvation to 3.7-fold for osmotic shock. The mini-Tn5 flanking DNA was cloned from the chromosome of MK114. The cloned DNA fragment exhibited carbon starvation activity, indicating that this fragment contains a carbon starvation-related promoter region. This region was partially sequenced. Possible physiological roles of Cst114 in a carbon sensing mechanism and in other stress responses are also discussed.

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