Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Enhancement of Human Lysozyme Secretion with Permeabilizing Agents from the Recombinant Saccharomyces cerevisiae

재조합 Saccharomyces cerevisiae에서 Permeabilizing Agent를 이용한 HLY의 분비촉진

  • Published : 1999.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

We have intended to accelerate the secretion of human lysozyme(HLY) with permeabilizing agents from the cultivated cells of the recombinant Saccharomyces cerevisiae. The five agents CaCl2, Tween 80, ethanol, Triton X 100, and cetyltrimethylammonium bromide(CTAB) were used as permeabilizing agents. Treatments of the yeast cell with CaCl2, Tween 80, and ethanol were effective to increase the secretion from the yeast cells. Especially, treatment of 10% ethanol increased the extracellular HLY activity by 38.6% at 30oC for 48 h in culture broth. But Triton X 100 and CTAB unexpectedly didn't play a role in increase of HLY secretion. Recovery of a foreign protein by permeabilizing agents is easier than by osmotic shock, and is less expensive than enzymatic digestion.

Keywords

References

  1. J. Mol. Biol. v.61 Immunochemical resemblance between human leukemia and hen egg white lysozyme and their ueduced carboxymethyl derivatives Armheim, N.;Sobel, J.;Canfield, R.
  2. Lysozyme Human leukemia lysozyme Canfield, R. E.;Collins, J. C.;Sobel, H. J.;Osserman, E. F.;Canfield, R. E.;Beychok, S.(eds.)
  3. Kor. J. Appl. Microbiol. Biotechnol. v.26 increased expression of a chemically synthesixed human lysoxyme gene in Saccharomyces cerevisiae Kim, K. W.;Choi, S. U.;Lee, S. C.;Paik, H. E.;Hwang, Y. I.
  4. Kor. J. Appl. Microbiol. Biotechnol. v.24 Effect of non-ionic surfactants on cloned glucoamylase production and secretion in recombinant yeast culture Cha, H. J.;Yoo, Y. J.
  5. Enzyme. Micorb. Technol. v.13 Permeabilization of baker's yeast by cethltrimethylammonium bromide for intracellular enzyme catalysis Gowda, L. R.;Bachhawat, N.;Bhat, S. G.
  6. Biotechnol. Lett. v.9 Permeabilization of yeast cells(Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide Joshi, M. S.;Gowda, L. R.;Bhat, S. G.
  7. J. Gen. Microbiol. v.136 Stimulation of exoprotein secretion by choline and Tween 80 in Trichoderma reesei QM 9414 corelates with increased activities of dolichol phosphate mannose synthase Kruszewska, J.;Palamarczyk, G.;Kubicek, C. P.
  8. J. Ferment. Bioeng. v.81 Effect of fatty acid saturation in membrane lipid bilayers on simple diffusion in the presence of ethamol at high concentrations Mizoguchi, J.;Hara, S.
  9. J. Ferment. Bioeng. v.83 Ethanol-induced alterations in lipid composition of Saccharomyces cerevisiae in the presince of exopenous fatty acid Mizoguchi, J.;Hara, S.
  10. J. Ferment. Bioeng. v.85 Permeability barrer of the yeast plasma membrane induced by ethanol Mizoguchi, J.;Hara, S.
  11. Enzyme Microb. Technol. v.12 Recorery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization Naglak, T. J.;Wang, H. Y.
  12. Kor. J. Appl. Microbiol. Biotechnol. v.19 Construction of an expression vector system with the GAP promoter in Saccharomyces cerevisiae Hwang, Y. I.;Seo, A. R.;Shim, S. K.;Chung, D. H.
  13. Anal. Biochem. v.128 Tuibidimetric determination of lysozyme with Micrococcus lysodeikticus colls : reexamination of reaction conditions Morsky, P.
  14. Kor. J. Appl. Microbiol. Biotechnol. v.23 Chemical synthesis of a human lysozyme gene and expression in Saccharomyces cerevisiae Kim, K. W.;Lee, S. C.;Hwang, Y. I.
  15. Gene v.43 Expression of synthetic human-lysozyme gene in Saccharomyces cerevisiae use of a synthetic chicken-lysozyme signal sequence for secretion and processing Jigami, Y.;Muraki, M.;Harada, N.;Tanaka, H.
  16. Digestion v.31 Effect of cthanol on the peptic and degradation of gastric mucus glycoprotein Laszewicz, W.;Solmiany, A.;Murty, V. L.;Liau, Y. H.;Slomiany, B. L.
  17. Biochem. J. v.273 Inhibition of proteolysis in the liver by chronic ethanol feeding Paso, A. R.;Hirsimaki, P.