Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Microdomain Formation in Phosphatidylethanolamine Bilayers Detected by $^2H$ NMR

  • Published : 1999.06.20
Download PDF
⟨ Previous Next ⟩

Abstract

In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet. This suggests the presence of two backbone conformations, the exchange between which is slow on an NMR time scale. The origin of the two conformations has been investigated in this work using saturated 1,2-diacyl-sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids.

Keywords

References

  1. Biophys. Acta v.944 Yoshikawa, W.;Akutsu, H.;Kyogoku, Y.;Akamatsu, Y.
  2. Acc. Chem. Res. v.20 Seelig, J.;Macdonald, P. M.
  3. Quart. Rev. Biophys. v.24 Bloom, M.;Evans, E.;Mouristen, O. G.
  4. Biochemistry v.31 Bazzi, M. D.;Youakin, M. A.;Nelsestuen, G. L.
  5. Biochemistry v.27 Wiedman, T. S.;Pates, R. D.;Beach, J. M.;Salmon, A.;Brown, M. F.
  6. Biochim. Biophys. Acta v.1113 Litsinger, D. C.;Huang, L.
  7. Biochem. Biophys. Acta v.466 Uehara, K.;Akutsu, H.;Kyogoku, Y.;Akamatsu, Y.
  8. Can. J. Biochem. Physiol. v.37 Bllgh, E. G.;Dyer, W. J.
  9. Synthetic Comm. v.20 Tomoi, M.;Kimura, Y.;Tokuyama, S.
  10. Biochemistry v.20 Gally, H. U.;Pluschke, G.;Overath, P.;Seelig, J.
  11. Biochemistry v.19 Wholegemuth, R.;Waespe-Sarcevic, N.;Seelig, J.
  12. J. Biol. Chem. v.252 Jakson, M. B.;Sturtevant, J. M.
  13. Biochim, Biophys. Acta v.598 Akutsu, H.;Akamatsu, Y.;Shindo,T.;Uehara, K.;Takahashi, K.;Kyogoku, Y.
  14. Biophys. Acta v.944 Epand, R. M.;Bottega, R.
  15. Proc. Natl. Acad. Sci. v.75 Albon, N.;Sturtevant, J. M.
  16. Proc. Natl. Acad. Sci. v.71 Tsong, T. Y.
  17. Biochem. Biophys. Acta v.1061 Gordeliy, V. I.;Ivkov, V. G.;Ostanevich, Yu. M.;Yaguzhinskij, L. S.
  18. Biochemistry v.21 Blume, A.;Wittebort, R. J.;Das Gupta, S. K.;Griffin, R. G.
  19. Biochim et Biophys. Acta v.857 Silvius, J. R.
  20. Biochemistry v.29 Fenske, D. B.;Jarrell, H. C.;Guo, Y.;Hui, S. W.
  21. Biochemistry v.31 Gawrisch, K.;Parsegian, V. A.;Hajduk, D. A.;Tate, M. W.;Gruner, S. M.;Fuller, N. L.;Rand, R. P.
  22. Proc. R. Soc. Lond. v.354 Elder, M.;Hitchcock, P.;Mason, R.;Shipley, G. G.