Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Studies on the Cytotoxicity of the Ethyl Acetate Soluble Sophora flavescens Ait. Extract against L1210 and $P388D_1$ Cells (III)

L1210 및 $P388D_1$ 세포에 대한 고삼 에틸 아세테이트 추출물의 세포독성에 관한 연구 (III)

  • Ryu, Hong-Sun (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Shin, Min-Kyo (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Yang, Eun-Yeong (Kunhil Pharmaceutical Co., Ltd.) ;
  • Cho, Hoon (Kunhil Pharmaceutical Co., Ltd.) ;
  • Chai, Kyu-Yun (Division of chemistry Technology & Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Kang, Kil-Ung (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baek, Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 류홍선 (원광대학교 한의과대학 본초학교실) ;
  • 신민교 (원광대학교 한의과대학 본초학교실) ;
  • 양은영 (건일제약(주) 중앙연구소) ;
  • 조훈 (건일제약(주) 중앙연구소) ;
  • 채규윤 (원광대학교 화학기술.생명과학부) ;
  • 강길웅 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 백승화 (원광대학교 한의학전문대학원 천연물학교실)
  • Published : 2000.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate cytotoxic effects of the roots of Sophora flavescens Ait. extracts on murine leukemia tumor cells lines $(P388D_1\;and\;L1210)$. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The comparison of $IC_{50}$ values of the ethyl acetate of Sophora flavescens Ait. extract in leukemia cell lines showed that their susceptibility to these extracts decreased in the following order : Adriamycin>Fr.4>Fr.5>Fr.3>Fr.1>Fr.2 by the MTT assay. These results suggest that the fraction 4 of the ethyl acetate soluble extract of Sophora flavescens Ait. may be a valuable choice for the studies on the treatment of murine leukemia cell lines.

Keywords