Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

The Effects of Rhus Extracts on The Cytotoxicity on Cancer Cells and E6 and E7 Oncogenes of Human Papillomavirus Type 16

옻 추출물의 세포독성 및 자궁 경부암 바이러스 암 유발인자 E6 와 E7의 작용에 미치는 효과

  • Cho, Young-Sik (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Joung, Ok (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Cho, Cheong-Weon (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Ae (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Shim, Jung-Hyun (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Kim, Kwang-Soo (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Hong-Soo (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Seung, Ki-Seung (Korea Food Research Institute) ;
  • Yoon, Do-Young (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology)
  • Published : 2000.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cervical cancer has been one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papillomaviruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, Rhus, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Rhus inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, Caski) and HaCaT keratinocytes in a dose response manner. In vitro binding assay and ELISA showed that Rhus inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Rhus inhibited the in vitro binding of E7 and Rb which essential tumor suppressor for the control of cell cycle. The level of mRNA for E6 was also decreased by Rhus while that of E7 mRNA was not changed. Our data suggested that Rhus inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas by HPV.

자궁 경부암은 매년 약 50만명 정도씩 사망하는 여성의 치명적인 사망원인의 하나이다. 인두유종 바이러스(HPV) 16형 및 18형과 자궁 경부암과의 긴밀한 관련성은 잘 알려져 있다. 옻 추출물 Rhus가 HPV 16형의 E6, E7 발암 유전자를 억제하는지 여부를 측정하였다. 이 Rhus는 자궁 경부암 세포주(C-33A, SiHa, Caski)와 HaCaT keratinocytes의 분열은 농도 의존적으로 억제하였다. In vitro binding assay와 효소면역측정법에 의하면 Rhus가 암 억제인자인 p53과 결합하여 분해 시키는데 필수적인 E6와 E6AP와의 결합을 억제할 뿐더러 암 억제인자 Rb와 E7과의 결합을 억제하였다. RT-PCR에 의하면 Rhus에 의해 E6 mRNA의 level이 감소하였으나 E7 mRNA는 변하지 않았음을 보여주었다. 이들 결과에 의하면 Rhus가 HPV 16형의 E6와 E7의 발암성을 억제함을 보여 주므로 HPV에 의해 유도된 자궁 경부암의 치료에 유효할 것으로 사료되어 좀 더 자세한 in vitro실험 등이 요구된다.

Keywords