The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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An Experimental Study on the Hepatoprotective Effect of Gokajisilsosiho-Tang

곡아지실소시호탕(穀芽枳實小柴胡湯)의 간보호작용(肝保護作用)에 관(關)한 실험적(實驗的) 연구(硏究)

  • Kim, Young-Jin (Department of Oriental Medicine Graduate School of Wonkwang University) ;
  • Kang, Dae-Geun (Department of Oriental Medicine Graduate School of Wonkwang University) ;
  • Lee, Jae-Ik (Department of Oriental Medicine Graduate School of Wonkwang University) ;
  • Kim, Kang-San (Department of Oriental Medicine Graduate School of Wonkwang University) ;
  • Kang, Byung-Ki (Department of Oriental Medicine Graduate School of Wonkwang University) ;
  • Cheon, Young-Sae (Department of Oriental Medicine Graduate School of Wonkwang University)
  • 김영진 (원광대학교 한의과대학 내과학교실) ;
  • 강대근 (원광대학교 한의과대학 내과학교실) ;
  • 이재익 (원광대학교 한의과대학 내과학교실) ;
  • 김강산 (원광대학교 한의과대학 내과학교실) ;
  • 강병기 (원광대학교 한의과대학 내과학교실) ;
  • 전영세 (원광대학교 한의과대학 내과학교실)
  • Published : 2000.10.30
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Abstract

This study was performed to elucidate the effects of Gokajisilsosiho-Tang(GJST) on the lactic dehydrogenase(LDH) release, cell viability and activity, lipid peroxidation, DNA synthesis and the changes of total protein synthesis and GSH changes in vivo and in vitro in rat cultured hepatocytes from hydrogen peroxide$(H_2O_2)$-induced injury. The GJST extract had not an effect on cytotoxicity in all experimental results. The treatment of GJST extract of $160{\mu}g/ml$, $320{\mu}g/ml$ showed the significant effect to decrease LDH leakage induced by t-BHP in cultured rat hepatocytes. The higher concentration of GJST extract than $160{\mu}g/ml$, showed the inhibitory effect on decreasing cell viability induced by t-BHP. The treatment of t-BHP to rat cultured hepatocytes resulted in a concentration dependent increase in TBARS, in the presence of GJST extract the production of TBARS induced by hydrogen peroxide was inhibited concentration dependently, significantly inhibited at $80{\mu}g/ml$ of GJST extract and above. The GJST extract simutaneously present with t-BHP prevented the loss of total protein and GSH in a concentration dependent manner. These results suggested that GJST extract may play a hepatoprotective role in oxidative damage induced by hydrogen peroxide and a therapeutic potential of inhibiting liver injury.

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References

  1. 古今醫統秘方大全 徐春甫
  2. 臨床と硏究 v.57 慢性肝炎に對する小柴胡湯桂枝茯笭丸の治療效果 牧坂泰治ほか
  3. 漢方醫學 v.7 柴胡湯およびサイコサポニソによる 慢性肝炎治療の試み 熊谷 明;山本昌弘
  4. Free Radical Biol. Med v.20 Ascorbyl Radical Formation in Patients with Sepsis. Effect of Ascorbate Loading Galley F.H.;J.M.;Webster, R.N.
  5. Life Sci. v.8 Endotoxin Depletes Ascorbate in the Guinea Pig Heart. Protective Effaces of Vitamins C and E Against Oxidative Stress Rojas, C.;Cadenas, S.;Herrero, A.;Mendez, J.;Barja, G.
  6. Exp. Toxicol. Pathol. v.5 Effect of Exogenous Antioxidant Enzymes and Antioxidants on the Sevelopment of Endotoxin-Induced Hepatocellular Necrosis in Rats Shibayama, Y.;Nakata, K.
  7. J. Mutr. Sci. Vitaminol. v.40 Antio xidative Effects of Processed Grain Food Minamiyama, Y.;Yoshikawa, T.;Tanigawa, T.;Takahashi, S.;Naito, Y.;Ichikawa, H.;Kondo, M.
  8. Alternatives to Animal Testing Improved drug metabo lizing capacity of hepatocytes cocu Itured with epithelial cells and maintained in a Perifusion system Gebhardt, R.;Reinhard, C.H.(ed.)
  9. J. Immunol. Methods v.65 Rapid colorimetric assay for cellular growth and survival Application to proliferation and cytotoxic assays Mosmann, T.
  10. Biochem. Med v.15 A simple fluorometric assay for lipoperoxide in blood plasma Yagi, K.
  11. Methods Enzy mol v.52 Microsomal lipid peroxidation. Buege, J.A.;Aust, S.D.
  12. Anal. Biochem v.27 Enzymic Method for quantitative determination of nano gram amouts of total and oxidized glutathione : Applications to mammalian blood and other tissues Tietze, F.
  13. Arch. Biochem. Biophys v.82 Tissue Sulfhydryl Groups. Ellman, G.L.
  14. Methods Enzymol v.186 Use of isolated perfused organs in hypoxia and jschemia/reperfusion oxidant stress Jaeschke, H.;Mitchell, J.R.
  15. B型慢性肝炎に對する小柴胡湯のHBe抗原${\cdot}$抗體系への果, 肝炎-增補C型肝炎 藤原硏司;持田智
  16. 慶熙韓醫大 論文集 v.2 膈下逐瘀湯 煎湯液이 CCl4로 誘發된 白鼠 肝損傷에 關한 硏究 田賢哲(外)
  17. 薺菜가 TAA 中毒 白鼠의 肝損傷에 미치는 影響 金漢燮
  18. 加減茵蔯蒿湯 木針液이 膽道結紮로 誘發된 肝損傷에 미치는 影響 李建穆
  19. Antioxidative and protective properties of extracts from leaves of the Artichoke(Cynara scolymus L.) against hydropeoxide induced oxidative stress in cultured rat hepatocytes. Gebhardt, R.
  20. Am. J. Physiol v.206 Supplemental fatty acids alter lipid peroxidation and oxidant injury in endothelial cells Hart, C.M.;Tolson, J.K.;Block, E.R.
  21. Biochem. Biophys. Res. Commun v.78 Age-dependent changes in rat liver microsomal and mitochondrial NADPH-dependent lipid peroxidation Player, T.J.;Mills, D.J.;Horton, A.A.
  22. Biochem. Physiol v.88 Gluthathione and lipid peroxidation in the aging rat Comp Farooqui, M.Y.;Day, W.W.;Zamorano, D.M.
  23. Chem. and Phys. of Lipid v.45 Lipid peroxides and diseases Yagi, K.
  24. Mech. Ageing Dev v.65 Effect of age-related lipid peroxidation on membrane fluidity and phospholipase Yu, B.P.;Suescun, E.;Yang, S.Y.
  25. J. Biol. Chem v.262 Age-related changes in oxidated proteins Oliver, C.N.;Ahn, B.;Meorman, E.J.;Goldstein, S.;Stadtman, E.R.