Pseudorabies virus(PRV)를 이용한 두침(頭針) 치료(治療) 기전(機轉)에 대한 신경해부학(神經解剖學的) 연구(硏究)

Neuroanatomical studies on the mechanism of scalp acupuncture therapy using the pseudorabies virus

  • 이태영 (우석대학교 한의과대학 경혈학교실) ;
  • 이창현 (우석대학교 한의과대학 경혈학교실) ;
  • 이상룡 (우석대학교 한의과대학 해부학교실) ;
  • 육상원 (우석대학교 한의과대학 해부학교실) ;
  • 이광규 (우석대학교 한의과대학 원전의사학교실) ;
  • 육태한 (우석대학교 한의과대학 병리학교실)
  • Lee, Tae-Young (Department of Meridianology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Meridianology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Sang-Ryoung (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Yuk, Sang-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Lee, Kwang-Gyu (Department of Classics, College of Oriental Medicine, Woosuk University) ;
  • Yuk, Tae-Han (Department of Oriental Pathology and Acupuncture, College of Oriental Medicine, Woosuk University)
  • 발행 : 2000.06.20

초록

본 실험은 pseudorabies 바이러스 (PRV) 의 Bartha strain 을 안면신경의 측두지, 하지를 지배하는 신경 (좌골신경) 및 상지를 지배하는 신경 (요골, 척골, 정중신경) 에 주입한 후 4 일간의 생존시간이 경과한 후 척수와 뇌를 적출하여 동결절편을 제작한 후 면역조직화학적 염색기법과 X-gal 조직화학 염색법을 시행하여 염색된 신경세포체를 척수와 뇌에 투사된 공통영역을 관찰하고 두침의 영역중 하나인 운동구와 사지와의 관계에 대한 실험적 증거를 제시하고자 시행하였다. 위의 실험에서 얻어진 결과는 아래와 같다. 1. 안면신경의 측두지, 하지를 지배하는 신경 (좌골신경) 및 상지를 지배하는 신경 (요골, 측골, 정중신경) 에서 투사된 공통된 영역은 척수에서 경수의 층판 1-IV, 흉수의 intermediolateral nucleus(IML), dorsal nucleus(D) 및 층판 X, 요수의 층판 IV, V, 천수의 층판 IV, V, IX, X 등의 영역에서 관찰되었고, 뇌줄기에서는 caudoventrolateral reticular nucleus(CVL), nucleus solitary tract(Sol), rostroventrolateral nucleus(RVL), area postrema(AP), raphe nuclei(raphe pallidus, raphe obscurus, raphe magnus), inferior olivary nucleus 의 등쪽부분 (gigantocellular reticular nucleus, Gi), Kolliker-Fuse nucleus(KF), central gray(CG), dorsal raphe nucleus (DR) and A5 영역에 표지된다. 또한 paraventricular hypothalamic nucleus(PRV) 와 lateral hypothalamic reticular nucleus(LH)에서도 관찰되고 locus coeruleus(LC) 와 subcoeruleus nuc!eus(SubCA) 에서도 관찰된다.

키워드

참고문헌

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