Isolation and Identification of Cellulose-Producing Bacteria

Microbial Cellulose 생산세균의 분리 및 동정

  • Published : 2000.06.01

Abstract

Extensive screening for cellulose-producing bacteria was done using differential media. Fifty seven strains were isolated totally from the fruits and the vinegar, respectively; the isolate A9 strain from apples was selected and examined to determine its taxonomical characteristics. The bacterium was identified as the genus Acetobacter sp_ based on morphological, cultural and biochemical properties. A9 strain produced acetic acid from ethanol and decomposed acetic acid to $CO_2$ and $H_2O$. They produced dihydroxyacetone from glycerol but did not produce y-pyrone from glucose and fructose. When A9 strain was cultivated statically in Hestrin and Schramm liquid medium(HS medium). thick cellulose pellicle was formed_ Higher cellulose production was obtained in the shaken culture using HS medium at 100 rpm.

자연계에 다양하게 존재하는 세균중 셀룰로오스 생산성이 우수한 균주를 분리 및 동정함으로서 새로운 생물자원을 확복하고자 하였다 사과, 포도, 식초 등의 시료로부터 총 57주의 셀룰로오스 생산균주를 분리하였다. 그중 사과에서 분리된 A9 균주를 공시균으로 선정하여 형태학적 배양적 및 생리생화학적 특성을 검토한 결과 Acetobacter 속으로 동정되어 편의상 Acetobacter sp. A9로 명명하였다 본 균주는 ethanol을 acetic acid로 산화시킨 후 다시 $CO_2$$H_2O$. 로 재산화시키는 특성을 가지고 있었다 또한 glycerol로부터 dihydroxyacetone을 생성할 수 있었으나 glucose 및 fructose로부터 ${\gamma}$-pyrone은 생성할 수 없었다. 본균주를 HS액체배지에서 정치배양했을 때 두꺼운 셀룰로오스 pellicle을 합성하였으며 교반배양에서 mass 형태의 셀룰로우스를 합성할 수 있었다.

Keywords

References

  1. The Prokaryotes v.3 Balows, A.;H. G. Truper;M. Dworkin;W. Harder;K. H. Schleifer
  2. Biotechnol. Lett. v.17 Mechanism of the film thickness increasing during the bacterial production of cellulose on non-agitated liquid media Borzani, W.;S. J de Souza
  3. J. Chem. Soc. v.49 An acetic ferment which forms cellulose Brown, A. J.
  4. European Patent EP 323717 Process for the production of microbial cellulose Byrom, D.
  5. Int. J. Sys. Bacteriol. v.20 The status of the generic name Gluconobacter De Ley, J.;J. Frateur
  6. Plant Cell v.7 Cellulose biosynthesis Delmer, D. P.;Y. Amor
  7. J. Gen. Microbiol. v.21 Cellulose production by Acetobacter acetigenum and other Acetobacter spp Dudman, W. F.
  8. Food Hydrocol. v.10 Isolation and partial characterization of cellulose produced by Acetobacter xylinum Embuscado, M. E.;J. N. BeMiller;J. S. Marks
  9. Appl. Environ. Microbiol. v.55 Synthetic medium for Acetobacter xylinum that can be used for isolation of auxotrophic mutants and study of cellulose biosynthesis Forng, E. R.;S. M. Anderson;R. E. cannon
  10. Bergey's Manual of Determinative Bacteriology(9th ed.) Holt, J. G.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams
  11. Bergey's Manual of Systematic Bacteriology v.1 Krieg, N. R.;J. G. Holt
  12. Biochemical Tests for Identification of Medical Bacteria Macfaddin, J. F.
  13. Microbiol. Rev. v.55 Cellulose biosynthesis and function in bacteria Ross, P.;R. Mayer;M. Benziman
  14. J. Gen. Microbiol. v.11 Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum Schramm, M.;S. Hestrin
  15. Can. J. Microbiol. v.24 Morphology, microstructure and development of colonics of Acetobacter xylinum Siwden, L. C.;J. R. Colvin
  16. Identification methods for microbiologists Skinner, F. A.;D. W. Lovelock
  17. TIBTECH v.16 Novel and estabilished applications of microbial polysaccharides Sutherland, I. W.
  18. Biosci Biotech. Biochem. v.59 Screening of bacterial cellulose-producing Acetobacter strains suitable for agitated culture Toyosaki, H.;T. Naritomi;A. Seto;M. Matsuoka;T. Tsuchida;F. Yoshinaga
  19. J. Gen. Microbiol. v.128 Cellulose-negative mutants of Acetobacter xylinum Valla, S.;J. Kjosbakken
  20. Appl. Environ. Microbiol. v.55 Alternative environmental roles for cellulose produced by Acetobacter xylinum Williams, W. S.;R. E. Cannon