Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Identification of Acebacter xylinum GS11 Producing Cellulose

Cellulose를 생산하는 Acetobacter xylinum GS11의 분리.동정

  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

산업적으로 유용한 미생물 유래 셀룰로오스를 생산 이용하기 위해 전국 각지 양조식초의 덧으로부터 세룰로오스의 생산성이 높고 조질의 균일성을 나타내는 균주를 분리하였다 분리균 GS11은 gram 음성이고 간균(0.6$\times$2.2~3.2 $\mu\textrm{m}$)의형태를 하고 있으며 편모를 가지고 있어 운동성을 보였다. 또한 세포내 지방산 조성은 다량의 불포화 지방산 {{{{ {C}_{18:1} }}}}과 포화지반산 {{{{ {C}_{16:0 } }}}}, {{{{ {C}_{14:0 } }}}} 이 대부분을 차지하였고 DNA 염기조성 (G+C) 함량은 58.4% 이였으며 ubiqunone 은 {{{{ { Q}_{10 } }}}}을 갖는 것으로 나타났다 이러한 형태학적 생리.생화학적 특성의 결과에 따라 본 균주는 Acetobacter xylinum GS11으로 동정되었다 A. xylinum, GS11 의배양기간 동안 셀룰로오스 생산성을 검토하고자 250mL 삼각플라스크에 균주를 접종하여 $30^{\circ}C$에서 12일간 정치배양하였다 그결과 기질인 glucose의 소비는 접종 후 급소하게 감소하여 셀룰로오스 생산에 이용되었으며 셀룰오스의 생산은 배양 9일 경에 2.8g/l로 최대의 생산량을 나타냈다.

Keywords

References

  1. J. Chem. Soc. v.49 An acetic ferment which forms cellulose Brown, A. J.
  2. Biochem. Biophys. Acta. v.82 Synthesis of cellulose by sarcina ventriculi Canale-Parola, E.;R. S. Wolfe
  3. Kor. J. Appl. Microbiol. Biotechnol. v.22 Isolation and characterization of cellulose producing Acetobacter xylinum KI strain Cha, Y. J.;K.J, Park;D. K. Kim;H. S. Chun;B. K. Lee;K. H. Kim;S. Y. Lee;S. J. Kim
  4. Manual for the identification of medical bacteria(2nd Ed.) Cowan and Steeel
  5. Biochem. J. v.56 Synthesis of cellulose by Acetobacter xylinum: 1. Micromethod for the determination of cellulose Hestrin, S.;M. Schramm
  6. Kor. J. Biotechnol. Bioeng. v.14 Production of high concentration cellulose by Acetobacter xylinum BRC5 in fed-batch culture Hwang, J. W.;C. S. Lee;S. h. Park;Y. R. Pyun
  7. Bergey's manual of systematic bacteriology v.1 Krieg, N. R.;J. G. Holt
  8. Mokchae Konghak v.20 Bacterial cellulose.-The possibility of raw material for paper making fiber-. Kuga, S.
  9. Biochemical tests for identification of medical bacteria(2nd ed.) Mac Faddin, J. F.
  10. J. Ferment. Bioengin. v.75 Production of cellulose from glucose by Acetobacter xylinum Masaoka, S.;R. Ohe;N. Sakota
  11. J. Bacteriol. v.154 Role of cellulose fibrils in Agrobacterium tumefaciens infection Matthysse, A.G.
  12. Kor. J. Biotechnol. Bioeng. v.8 Some properties of Acetobacter sp. isolated from traditional fermented vinegar Park, J. P.;S. J. Kim;J. C. Ryu;B. S. Pyo;S. W. Kim
  13. Kor. J. Appl. Microbiol. Biotechnol. v.25 Microbial cellulose fermentation by Acetobacter xylinum BRC5 Park, S. H.;Y. K. Yang;J. W. Hwang;C. S. Lee;R. Pyun
  14. Transaction v.50 High bacterial cellulose production by Acetobacter xylinum ATCC 10245 in a new culture medium with a sulfite pulping waste fraction Premjet, S.;Y. Ohtani;K. Sameshima
  15. Microbiol v.17 Biogenesis of bacterial cellulose Robert, E. C.;M. A. Steven
  16. Microbiol Reviews Cellulose biosynthesis and function in bacteria Ross, P.;R. Mayer;M. Benziman
  17. FEMS Microbiol. Lett. v.25 Determination of DNA base composition by reversed-phase high performance liquid chromatography Tamaoka, J.;K. Komagata
  18. J. Ferment. Bioeng. v.84 Cellulose production by acetic acid-resistant Acetobacter xylinum Toda, K.T. Asakura;M. Fukaya;E. Entani;Y. Kawamura
  19. Biosci. Biotech. Biochem. v.59 Addition of a small amount of an endoglucanase enhances cellulose production by Acetobacter xylinum Tonouchi, N.;N. Tahara;T. Tsuchida;F. Yoshinaga;T. Beppu;S. Horinouchi
  20. Biosci. Biotech. Biochem. v.59 Screening of bacterial cellulose-producing Acetobacter strains suitable for agitated culture Toyosaki, H.;T. Naritomi;A. Seto;M. Matsuoka;T. Tsuchida;F. Yoshinaga
  21. Int. J. Syst. Bacteriol. v.36 Acetobacter methanolicus sp. Nov., and acidophlilic facultatively methylotrophic bacterium Uhlig, H.;K. Karbaum;A. Steudel
  22. J. Gen. Appl. Microbiol. v.27 The cellular fatty acid composition in acetic acid bacteria Yamada, Y.;M. Nunoda;T. Ishikawa;Y. Tahara
  23. J. Mat. Sci. v.24 The structure and mechanical properties of sheets prepared from bacterial cellulose Yamanaka, S.;K. Watanabe;N. Kitamura;M. Iguchi;S. Mitsuhashi;Y. Nishi;M. Uryu