Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Immobilization of Cyclodextrin Glucanotrasferase on Amberline IRA-900 for Biosynthesis of Transglycosylated Xylitol

  • Kim, Pan-Soo (Dipartment of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Taegu 702-701) ;
  • Shin, Hyun-Dong (Depatment of Genetic Engineering, College of Natural Siences, kyungpook national university, taegu 702-701) ;
  • Park, Joong-Kon (Depantment of Chemical Engineering, College of Engineering, Kyungpook National University, Taegu 702-701) ;
  • Lee, Young-Hyun (Depatment of Genetic Engineering, College of Natural Siences, kyungpook national university, taegu 702-701)
  • Published : 2000.06.01
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Abstract

Cyclodextrin glucanotransferase (CGTasa) from Thermoanaerobacter sp. was adsorbed on the ion exchange resin Amberlite IRA-900. The optimum conditions for the immobilization of the CGTase were pH6.0 and 600 U CGTase/g resin, and the maximum yield of immobilization was around 63% on the basis of amount ratio of the adsorbed enzyme to intial amount in the solution. Immobilixation of CGTase shifted the optimum temperature for the enzyme to peoduce transglycosylated xylitol from 7$0^{\circ}C$ to 9$0^{\circ}C$ and improved the thermal stability of immobilized CGTase, especially after the addition of soluble starch and calcium ions. Transglycosylated xylitol was continuoncly produced using immobilized CGTase in the column type packed bed reactor, and the operating conditions for maximum yield were 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) xylitor as the glycosyl acceptor, 20mL/h of medium fiow rate, and 6$0^{\circ}C$. The maximum yield of transglycosylated xylitol and productivity were 25% and 7.82 g.L-1.h-1, respectively. The half-life of the immobilized CGTase in a column type packed bed reactor was longer than 30 days.

Keywords

References

  1. Alternative Sweetners Xylitol Bar, A.;L. O. Nabors(ed.);R. C. Gelardi(ed.)
  2. Antimicrob. Agents. Chemother. v.39 Effect of xylitol on growth of nasopharyngeal bacteria in vitro Kontiokari, T.;M. Uhari;M. Koskela
  3. J. Jpn. Soc. Starch Sci. v.34 Studies on cyclomaltodextrin glucanotransferase and coupling sugar Okada, S.
  4. J. Biochem. v.79 Studies on cyclodextrin glycosyltransferase Ⅳ. Enzymatic synthesis of 3-O-α-D-glucopyranosyl-L-sorbose and 4-O-α-D-glucopyranosyl-D-xylose using cyclodextrin glycosyltransferase Kitahata, S.;S. Okada
  5. Agric. Biol. Chem. v.55 Synthesis of 2-O-α-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus sterothermophilus Aga, H.;M. Yoneyyama;S. Sakai;I. Yamamoto
  6. Biosci. Biotech. Biochem. v.58 Transglycosylation to hesperidin by cyclodextrin glucanotransferase from an alkalophilic Bacillus species and in alkaline pH and properties of hesperidin glycosides Kometani, T.;Y. Terada;T. Nishimura;H. Takii;S. Okada
  7. Denpun Kagaku v.38 Application of cyclodextrin glucanotransferase Okada, S.;S. Kitahata;M. Shiosaka;H. Bunya;M. Kubota;S. Sakai;Y. Sujisaka
  8. Biotechnol. Lett. v.13 Synthesis of novel sugars, ologoglucosyl-insoitol, and their growth stimulating effect for Biffidobacterium Sato, M.;T. Matsuo;N. Orita;Y. Yagi
  9. Biotechnol. Lett. v.14 Synthesis of glucosyl-inositol using a CGTase, isolation and charcterization of positional isomers, and assimilation profile for intesinal vacteria Sato, M.;K. Nakamura;H. Nagano;Y. Yagi;K. Koizumi
  10. J. Microbiol. Biotechnol. v.7 Synthesis of glucosyl-sugar alcohols using cyclodextrin glucosyltransferase and structural identification of glucosyl-maltitol Kim, T. K.;D. C. Park;Y. H. Lee
  11. Kor. J. Appl. Microbiol. Biotechnol. v.26 Synthesis of transglycosylated xylitol using cyclodextrin glucosyltransferase and its stimulating effect on growth of Biffidobacterium Kim, T. K.;D. C. Park;Y. H. Lee
  12. Biotechnol. Bioeng. v.19 Production of schardinger β-dextrin by soluble and immobilized cyclodextrin glycosytransferase of an alkalophilic Bacillus sp Nakamura, N.;K. Horikoshi
  13. Biotechnol. Bioeng. v.26 no.38-2 Immobilized cyclodextrin glucanotransferase of an alkaophilic Bacillus sp Kato, T.;K. Hrikoshi
  14. J. Jpn. Soc. Atarch Sci. v.33 The continuous reaction of cyclodextrin formation by the column method using the immobilized enzyme on ion exchange resins Hitosh, H.;K. Hara;N. Kuwahara;S. Sakai;N. Yamamoto
  15. J. Ferment Bioeng. v.77 Immobilization of cyclodextrin glucanotransferase on capillary membrane Okada, T.;M. Ito;K. Hibino
  16. J. Microbiol. Biotechnol. v.1 Evaluation ofimmobilization methods for cyclodextrin glucano-transferase and characterization of its enzymatic properties Lee, Y. H.;S. H. Lee;H. D. Shin
  17. J. Microbiol. Biotechnol. v.1 Performance of column type bioreactor packed with immobilized cyclodextrin glucanotransferase for cyclodextrin production Lee. Y. H.;S. H. Lee;H. D. Shin
  18. Kor. J. Appl. Microbiol. Biotechnol. v.25 Immobilization of cyclodextrin glucanotransferase and its reaction characteristics regarding transglycosylated stevoside production In, M. J.;D. C. Kim;H. J. Chae;K. S. Choi;M. H. Kim
  19. J. Ferment Bioeng. v.71 Purification and properties of Bacillus coagulans cylcodextrin glucanotransferase Akimaru, K.;T. Yagi;S. Yamamoto
  20. Agri. Biol. Chem. v.38 Action of cyclodextrin glucanotransferase from Bacillus megatrium strain NO. 5 on starch Kitahata, S.;S. Okada
  21. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  22. Denpun Kagaku v.39 Thermoanaerobacter sp. CGTase: its properties and application Norman, B. E.;S. T. Jorgensen
  23. J. Mol. Biol. v.256 Crystal structure at 2.3Å resolution and revised nucleotide sequence of the Thermoanaerobacterium thermosulfurigenes EM1 Knegtel, R. M. A.;R. D. Wind;H. J. Rozeroom;K. H. Kalk;R. M. BuitelaarlL. Dijkhuizen;B. W. Dijkstra
  24. Appl. Environ. Microbiol. v.61 Cyclodextrin formation by thermostable α-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassifycation of the enzyme as a cyclodextrin glucosyltransferase Wind, R. D.;W. Liebl;R. M. Buitelaar;D. Penniga;A. Spreinat;L. Dijkhuizen;H. Bahl
  25. J. Appl. Glycosci. v.42 Action of cyclodextrin producing enzyme (CGTase) and diglucosyl-cyclodextrin Kobayashi, S.;N. Watanabe;K. Nakashima;M. Shiota;T. Yatake
  26. Appl. Biochem. Biotechnol. v.30 Enhanced conversion of starch to cyclodextrin in ethanolic solution by Bacillus circulans var alkalophilus cyclomaltodextrin glucanotransferase Mattsson, P.;T. Korpela;S. Paavilainen;M. Makela