한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제29권2호
  • /
  • Pages.307-311
  • /
  • 2000
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

우리밀의 면역증강능 규명

Screening of Immune-Enhancing Substance(s) from Korean Wheats

  • 최면 (강원대학교 축산가공학과) ;
  • 박재봉 (한림대학교 의과대학 생화학교실) ;
  • 김현숙 (강원대학교 한국영양과학연구소)
  • Choe, Myeon (Dept. of Animal Products Science, Kangwon National University) ;
  • Park, Jae-Bong (College of Medicine, Hallym University) ;
  • Kim, Hyun-Sook (Institute of Korea Nutritional Science, Kangwon National University)
  • 발행 : 2000.04.01
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초록

The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats(Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones(Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA(ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05mg amout of EA extract added to J774 cells.

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