Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Effects of Water-Soluble Extract from Leaves of Morus alba and Cudrania tricuspidata on the Lipid Peroxidation in Tissues of Rats

뽕나무(Morus alba) 및 꾸지뽕나무(Cudrania tricuspidata) 잎의 수용성 추출물이 흰쥐 각 조직중의 지질 과산화물 함량에 미치는 영향

  • 차재영 (동아대학교 생명자원과학부) ;
  • 김현정 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학부)
  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

뽕나무 (Morus alba) 및 꾸지뽕나무(Cudrania tricuspidata) 잎으로부터 추출한 수용성 물질을 0.5% 콜레스테롤 투여 식이에 1%(w/w) 수준으로 첨가하여 각 조직에서의 지질 과산화물 생성정도를 나타내는 TBARS 측정법으로 항산화 활성을 검토하였다. 흰쥐의 체중 및 식이 섭취량은 실험군 간의 유의적인 차이는 없었다. 간장 및 뇌 조직 중량은 뽕잎 추출물군에서 증가하였다. 신장의 지질 과산화물 함량은 대조군에 비해서 뽕잎 추출물군에서 유의적으로 감소하였다. 뇌 및 심장의 지질 과산화에서 유의적으로 감소하였다. 뇌 및 심장의 지질 과산화물 함량은 대조군에 비교해서 꾸지뽕잎 추출물군에서 유의적으로 증가하였다. 간장 및 비장의 경우에는 각 실험군 간에 유의적인 차이가 없었다. 각 조직의 microsomes 분획으 지질 과산화물은 심장과 비장에서 뽕잎 및 꾸지뽕잎 추출물군에서 감소하였으나, 다른 조직에서는 현저한 차이가 없었다. 한편, 각 조직의 microsomes 분획을 얻어서 Fe2+/ascorbate로 지질 과산화물을 유도하기 위하여 37$^{\circ}C$에서 한시간 반응을 시킨 결과, 뽕잎 및 꾸지뽕잎 추출물군에서 약 절반 정도 감소하였으며, 상대적 증가량은 뇌조직 microsomes에서 현저하였다. 본 실험의 결과 신장조직 및 간장 microsome막 지질 과산화물 함량은 뽕잎 및 꾸지뽕잎 수용성 추출물에 의해 감소되었으며, 뇌 및 심장조직의 지질 과산화물 함량은 꾸지뽕잎 수용성 추출물에 의해 증가됨으로써 봉잎 추출물간의 차이를 나타내었다.

Keywords

References

  1. Annual report on the cause of death statistics
  2. Annu. Rev. Pharmacol. Toxical v.16 Chemicals, drugs and lipid peroxidation Plaa, G.L.;Witschi, H.
  3. Alcohol. v.25 Ethanol induced lipid peroxidation and oxidative stress in extrahepatic tissues Alordmann, R.;Ribierre, C.;Rouach, H.
  4. J. Korean Soc. Food Sci. Nutr. v.28 Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata Cha, J.Y.;Kim, H.J.;Chung, C.H.;Cho, Y.S.
  5. Kor. J. Pharmacogn. v.26 Effects of the fraction from the leaves, fruits, stems and roots of Cudrania tricuspidata and flavonoids on lipid peroxidation Park, J.C.;Choi, J.S.;Choi, J.W.
  6. Dehanshikmuldogam Lee, C.B.
  7. Jungyakdesajon. 2nd ed. Kangjoshineuihakwon
  8. Planta Medica v.51 Components of root bark of Cudrania tricuspidata 3. Isolation and structure studies on the flavonoids Fujimoto, T.;Nomura, T.
  9. J. Korean Soc. Food Sci. Nutr. v.22 Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau Kim, S.H.;Kim, N.J.;Choi, J.S.;Park, J.C.
  10. Acta Chem. Scand[B] v.31 The crystal structure of cudranone, 2,6,3’-trihydroxy-4-methoxy-2’-(3-methyl-2-butenyl)-I, a new antimicrobial agent from Cudrania chochinchinensis Ottersen, T.;Vance, B.;Doorenbos, N.J.;Chang, B.L.;El-Feraly, F.S.
  11. J. Ethnopharomacol. v.44 Effects of anti-lipid peroxidation of Cudrania cochinchinensis var. gerontogea Chang, C.H.;Lin, C.C.;Hattori, M.;Namba, T.
  12. Akugaku Zasshi v.115 Hypoglycemic activity and mechanisms of extracts from mulberry leaves (folium mori) and cortex mori radicis in streptozotocin-induced diabetic mice Chen, F.;Nakashima, N.;Kimura, I.;Kimura, M.
  13. J. Korean Soc. Food Sci. Nutr. v.27 Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia in rats Kim, S.Y.;Lee, W.C.;Kim, H.B.;Kim, A.J.;Kim, S.K.
  14. J. Inorganic. Biochem. v.14 The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid Wong, S.F.;Holliwell, B.;Richimond, R.;Skowroneck, W.R.
  15. Biometrics v.1 Multiple range and multiple F tests Duncan, D.B.
  16. J. Korean Soc. Food Sci. Nutr. v.28 Effects of total dietary restriction on the contents of thiobarbituric acidreactive substance and antioxidant enzymes in the liver and kidney of rats Park, P.S.;Koh, C.N.;Park, J.Y.
  17. J. Korean Soc. Food Sci. Nutr. v.27 Effects of green tea catechin on the microsomal mixed function oxidase system of kidney and brain in streptozotocin-induced diabetic rats Lee, S.J.;Shin, J.Y.;Cha, B.G.
  18. Arch. Environ. Health. v.24 Cadmium and zinc binding in mammalian liver and kidney Shaikh, Z.A.;Lucis, O.J.
  19. Diabetes Res. Clin. Pract. v.45 Oxidative damage to mitochondrial DNA and its relationship to diabetic complications Suzuki, S.;Hinokio, Y.;Komatu, K.;Ohtomo, M.;Onoda, M.;Hirai, S.;Hirai, A.;Chiba, M.;Kasuga, S.;Akai, H.;Toyota, T.
  20. J. Am. Diet Assoc. v.64 Lipid oxidation and fatty acid changes in beef combined with vegetables and textured vegetable protein Sangor, M.R.;Pratt, D.E.
  21. Annual Meeting of Korean Soc. Food Sci. Technol. Antioxidative effects and isolation of antioxidative compound from various Mulberry leaves in Korean Chung, S.K.;Kim, Y.C.;Park, S.W.
  22. New Food Industry v.39 Functional food of mulberry and silk worm Hirabayashi, K.
  23. J. Nutr. Sci. Vitaminol. v.38 Japanese soybean miso scavenges free radicals and inhibits lipid peroxidation Santiago, L.A.;Hiramatsu, M.;Mori, A.
  24. Biosci. Biotechnol. Biochem. v.63 Antioxidative effects of turmeric, rosemary and capsicum extracts on membrane phospholipid peroxidation and liver lipid metabolism in mice Asai, A.;Nakagawa, K.;Miyazawa, T.
  25. Biochem. Pharmacol. v.26 Inhibitory action of silymarin of lipid peroxidation formation in rat liver mitochondria and microsomes Bindol, A.;Carallin, L.;Silipandri, N.
  26. Biochem. J. v.204 Spin-trapping studies on the freeradical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat Albano, E.;Lott, K.A.;Slater, T.F.;Stier, A.;Symons, M.C;Tomasi, A.
  27. Br. J. Clin. Pharmacol. v.20 Effect of trimetazidine on membrane damage induced by oxygen free radicals in human red cells Maridonneau-Parini, I.;Harpey, C.
  28. Ann. Med. v.23 Effect of antioxidants on oxidative modification of low density lipoprotein Esterbauer, H.;Huhl, H.;Dieber-Rotheneder, M.;Waeg, G.;Rabl, H.
  29. Biochem. Pham. v.42 Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives Laughton, M.J.;Evana, P.J.Moroney, M.A.;Hoult, J.R.S.;Halliwell, B.
  30. Yakhak Hoeji v.36 Constituents of Cudrania tricuspidata in Korea Pack, J.C.;Young, H.S.;Choi, J.S.
  31. J. Korean Soc. Food Sci. Nutr. v.28 Effect of potato polyphenolics on lipid peroxidation in rats Cha, J.Y.;Cho, Y.S.