Effect of Seatangle and Seamustard Intakes on Carcinogen Induced DNA Adduct Formation and the Absorption of Calcium and Iron

다시마와 미역의 섭취가 발암물질에 의한 DNA 손상과 칼슘 및 철 흡수에 미치는 영향

  • 성미경 (숙명여자대학교 식품영양학과)
  • Published : 2000.10.01

Abstract

A number of epidemiological studies has indicated lifestyles including dietary habits are closely related to the development of certain forms of cancer. These findings have led several investigators to identify the ways in which these factors mdulate the risk of cancer. Seaweeds are rich sources of non-digestible polysaccharides which possibly posses physiological functions. In vitro studies showed several components in seaweeds inhibit tumor cell growth and mutagenicity of known food mutagens. On the other hand non-digestible polysaccharides of different food sources negatively affect mineral nutrition by decreasing mineral absorption. The objectives of this study was to investigate the effect of major seaweed intake on azoxymethane(AOM) - induced DNA damage a known cancer initiation step and on apparent absorption of calcium and iron. To accomplish these objectives twenty five ICR mice were divided into five groups and fed one of the following diets for 10 days : control diet d, diet containing 10% water-soluble fraction of seamustard or seatangle diet containing 10% water-insoluble fraction of seamustard or seatangle. AOM was injected 6 hours before sacrifice and N7-methylated guanines from the colonic DNA were quantified using a gas chromatography -mass spectroscopy. Fecal samples were collected on days 4 and 8. Caclium and iron contents of the diets and feces were analyzed using an atomic absorption spectrophotometry to determine the apparent absorption of these minerals. Results are as follows. AOM-induced guanine methylation of colon was decreased in animals fed diets containing water-soluble fractions of seamustard or seatangle compared to those in animals fed control diet although only the seatnagle fed group showed statistically significant effect. Apparent calcium absorption was significantly reduced in animals fed diets containing water-insoluble fractions of seaweeds. Iron absorption was significantly decreased and negatively balanced in animals fed diets containing water-insoluble fractions of both seaweeds, and water-soluble fraction of seatangle. In conclusion, seamustard and seatangle intakes may effectively prevent colon tumorigenesis by reducing a carcinogen-induced DNA damages, and more mechanistic studies on possible role of seaweeds on carcinogenesis are required. Also, adverse effects of seaweed diets cintaming a large amount of polysaccharides on mineral nutrition should be carefully monitored.

References

  1. Annual Report of the Central Caner Registry in Korea The Ministry of Health & Welfare
  2. Kor Soybean Digest v.13 The anticarcinogenic properties of soybean Sung MK
  3. Cancer Res v.51 Chemical and physical carcinogenesis: advances and perspectives for the 1990's Harris CC
  4. Drug Metab Rev v.26 The multistage nature of chemically induced hepatocarcinogenesis in the rat Pitot HC;Dragon YP
  5. Mutat Res v.365 DNA adducts: biological markers of exposure and potential application to risk assessment La DK;Swenberg JA
  6. Nutr Res Rev v.7 Anticarconogenic factors in plant foods: a new class of nutrients? Johnson IT;Wiliamson G;Musk SRR
  7. National Nutrition Survey Report The Ministry of Health & Welfare
  8. Am J Clin Nutr v.30 Why a new term for dietary fiber? Trowell H
  9. Nutr Rev v.35 The definition and analysis of dietary fibre Southgate DA
  10. Br J Nutr v.75 In vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds Michel C;Lahaye M;Bonnet C;Mabeau S;Barry J
  11. Biosci Biotech Biochem v.56 Effect of pH on the in vitro absorption of mutagens to dietary fibers Chiharu N;Tadashi N;Toshimara Y
  12. J Sci Food Agri v.66 Identification of antimutagenic activities in the extract of an edible brown algae. Hijikia fusiforme(Hijiki) by ume gene expression system in Salmonella typhimurium(TA 1535/pSK 1002) Yasuji O;Kiyoka HO
  13. J Kor Fish Soc v.31 Antimutagenic and antimicrobial effect of ethanol extracts from sea-mustard and sea-tangle Oh CK;Oh MC;Kim SH;Lim SB;Kim SH
  14. Kor J Food Sci Nutr v.27 Elucidation of anti-tumor initiator and promoter derived from seaweed-4: desmutagenic principles of Ecklonia stolonifera extracts against carcinogenic heterocyclic amines Park YB;Ahn JK;Yoo SJ;Park DC;Kim IS;Park YH;Kim SB
  15. J Kor Fish Soc v.31 Elucidation of anti-tumor initiator and promoter derived from seaweed-2: Investigation of seaweed extracts suppressing mutagenic activity of PhIP and MeIQx Park YB;Kim IS;Yoo SJ;Ahn JK;Lee TG;Park DC;Kim SB
  16. Hematol Oncol Clin North Am v.5 Dietary fiber and cancer prevention Shankar S;Lanza E
  17. Lancet v.1 no.8065 Calcium binding by dietary fibre James WP;Branch WJ;Southgate DA
  18. Am J Clin Nutr v.30 Binding of zinc and iron to wheat bread, wheat bran, and their components Ismail-Beigi F;Faraji B;Reinhold JG
  19. J Nutr v.110 Effect of Dietary fiber on mineral absorption in growing rats Harmuth-Hoene AE;Schelenz R
  20. Am J Clin Nutr v.34 Effect of cellulose incorporation in a low fiber diet on fecal excretion and serum levels of calcium, phosphorus and iron in adolescent girls Godara R;Kaur AP;Bhat CM
  21. J Kor Soc Food Sci Nutr v.27 A Study on nutritional characteristics of common Korean dietary fiber rich foods Oh HI;Ly SY
  22. Biochem Biophys Res Commun v.170 Mutiple mitochondrial DNA deletion exist in cardiomyocytes of patients with hypertrophic or dilated cardiomyopathy Ozawa T;Tanaka M;Sugiyama S;Hattori K;Ito T;Ohno K;Takahashi A;Sato W;Takada G;Mayumi B
  23. Metabol v.26 The effects of two dietary fiber supplements on gastrointestinal transit, stool weight and frequency, bacterial flora, and fecal bile acids in normal subjects Baird IM;Walters RL;Davies PS;Hill MJ;Drasar BS;Southgate DA
  24. Am J Clin Nutr v.68 Mechanisms by which wheat bran and oat bran increase stool weight in humans Chen HL;Haack VS;Janecky CW;Vollendorf NW;Marlett JA
  25. Lancet v.1 no.7846 Effect of bran on blood lipids and calcium Heaton KW;Pomare EW
  26. Am J Clin Nutr v.32 The effect of meat protein and dietary fiber on colonic function and metabolism Cummings JH;Hill MJ;Jivraj T;Houston H;Branch WJ;Jenkins DJA
  27. Am J Clin Nutr v.33 Influence of refined cellulose on human bowel function and calcium and magnesium balance Slavin JL;Marlett JA
  28. Trends Pharmacol Sci v.13 Metabolic polymorphism affecting activation of toxic and mutagenic arylamines Boch KW
  29. Cancer Epidemiol Biomarkers Prev v.3 Rapid metabolic phenotypes for acetyltransferase and cytochtome P450 1A2 and putative exposure of food-borne heterocyclic amines increase the risk for colorectal cancer or polyps Lang NP;Butler MA;Massengill J;Lawson M;Stotts RC;Houser-Jensen M;Kadlubar FF
  30. J Ferment Bioengineering v.76 Bacterial alginate lyase: enzymology, genetics and application Murata K;Inose T;Hisano T;Abe S;Yonemoto Y;Yamashita T;Takagi M;Sakaguchi K;Kimura A;Imanaka T
  31. Eur J Cell Biol v.74 Fucans, sulfated polysaccharides extracted from brown seaweeds, inhibit vascular smooth muscle cell proliferation. II. Degradation and molecular weight effect Logeart DP;Prigent-Richard S;Boisson-Vidal C;Chaubet F;Durand P;Jozefonvicz J;Letourneur D
  32. Jpn J Cancer Res v.86 Effects of apple pectin in fecal bacterial enzymes in azoxymethane-induced rat colon carcinogenesis Ohkami H;Tazawa K;Yamashita I;Shimizu T;Murai K;Kobashi K;Fujimaki M
  33. Cancer Res v.41 Effect of two kinds of pectin and guar gum on 1,2-dimethylhydrazine inhibition of colon tumors and on fecal β-glucuronidase activity in the rat Bauer HC;Asp N;Dahlqvist A;Fredlund PE;Nyman M;Oste R
  34. Carcinogenesis v.5 Inhibitory effect of lemon grass(Cymbopogon citratus Stapf) on formation of azoxymethane-induced DNA adducts and aberrant crypt foci in the rat colon Suaeyun R;Kinoouchi T;Arimochi H;Vinitketkumnuen U;Ohnishi Y
  35. Mutat Res v.350 Studies on the role of specific dietary fibres in protection against colorectal cancer Freguson LR;Harris PJ
  36. Bull Kor Fish Soc v.26 Inhibitory action of natural food components on the formation of carcinogenic nitrosamine Ahn BW;Lee DH;Yeo SG;Kang JH;Do JR;Kim SB;Park YH
  37. Kor J Nutr v.28 A Study in Ca Metabolism of college women eating usual Korean diet Yu CH;Hong HO
  38. Kor J Nutr v.21 A Study on Ca,P and Fe excretions in healthy adult Korean in their usual diet Lee LH;Lee IY;Roh YH;Paik HY;Kim KS;Cho JH
  39. J Food Sci v.51 In vitro estimation of the potential bioavailability of calcium from sea mustard(Undaria pinnatifida), milk, and spinach under simulated normal and reduced gastric acid conditions Kim H;Zemel MB
  40. J Nutr v.129 Nondigestible carbohydrates and mineral bioavailability Greger JL
  41. Am J Physiol v.266 Evidence that bile salt are important for iron absorption Sanyal AJ;Hirsch JI;Moore EW
  42. Br J Haematol v.45 Biliary iron excretion in rats following pyridoxal isonicotinoyl hydrazone Cikrt M;Ponka P;Necas E;Neuwirt J
  43. J Nutr v.126 Iron deficiency anemia among Alaska Natives may be due to fecal loss rather than inadequate intake Petersen KM;Parkinson AJ;Nobmann ED;Bulkow L;Yip R;Mokdad A