Korean Journal of Veterinary Research (대한수의학회지)
- Volume 40 Issue 1
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- Pages.86-93
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Expression of the C-terminal of 34kDa protein of Mycobacterium paratuberculosis
Mycobacterium paratuberculosis의 34kDa C-terminal 단백질의 발현
- Kim, Doo (Department of Veterinary Medicine, Kangwon National University) ;
- Park, Hyung-wook (Department of Veterinary Medicine, Kangwon National University)
- Received : 2000.01.08
- Published : 2000.03.25
Abstract
Paratuberculosis (Johne's disease), a chronic enteritis produced by Mycobacterium paratuberculosis, affects a large proportion of ruminants in all continents and causes important economic losses. The identification of well-characterized and species-specific components of M paratuberculosis would provide the means to improve the specificity and sensitivity of immunodiagnostic assays for Johne's disease. The aims of this study were to express the recombinant C-terminal of 34kDa protein (rC34P) of M paratuberculosis in E coli and to investigate the effectiveness of this protein in detecting antibodies to the native protein in sera from paratuberculosis infected cattle. The C-terminal of the gene encoding the 34kDa protein was amplified by polymerase chain reaction from the chromosomal DNA of M paratuberculosis (ATCC 19698) and cloned into vector pGEX-4T-2. Then, cloned plasmid was transformed into E coli DH5