Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Inhibitory Effects of the Methanol Extract of Houttuynia cordata $T_{HUNB}$ against Cadmium Induced Cytotoxicity (V)

어성초 메탄올 추출물의 카드뮴에 대한 독성억제효과(V)

  • Lee, Jeong-Ho (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jeong, Seung-Il (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • You, Il-Soo (Department of Industrial Chemistry, Iksan College) ;
  • Kim, Shin-Kee (Department of Environmental Horticulture, Iksan College) ;
  • Lee, Ki-Nam (Department of Industrial Oriental Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Baek, Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 이정호 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 정승일 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 유일수 (익산대학 공업화학과) ;
  • 김신기 (익산대학 환경원예과) ;
  • 이기남 (원광대학교 한의학전문대학원 산업한의학교실) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 백승화 (원광대학교 한의학전문대학원 천연물학교실)
  • Published : 2001.03.30
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Abstract

This study was conducted to investigate the antitoxic agent in methanol extract of Houttuynia cordata $T_{HUNB}$. Detoxication effects By H. cordata $T_{HUNB}$ extract increased in proportion to the extract concentrations. When 40 mg/kg dosage of H. cordata $T_{HUNB}$ extract was administered, it showed the highest antitoxic effects in metallothionein induction. After the extract treatment, body weights generally increased in proportion to the extract concentrations. From the above results, H. cordata $T_{HUNB}$ extract increased metallothionein concentrations and decreased the toxicity of cadmium in rats. In vitro the antitoxic activity of methanol extract of H. cordata $T_{HUNB}$ on NIH 3T3 fibroblasts was evaluated by the MTT {3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells, $10^{-2}\;mg/ml$ Concentrations of H. cordata $T_{HUNB}$ extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that H. cordata $T_{HUNB}$ extract retains a potential antitoxic activity.

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