Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cultivation of Transgenic Nicotiana tabacum Suspension Cells in Bioreacters for the Production of mGM-CSF

  • Lee, Sang-Yoon (Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University) ;
  • Won Hur (Department of Biotechnology, Kangwon National University) ;
  • Cho, Gyu-Heon (Department of Chemical Engineering, Kangwon National University) ;
  • Kim, Dong-Il (Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University)
  • Published : 2001.01.01
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Abstract

Transgenic Nicotiana tabacum cells were cultivated for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred tank bioreactor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor, 9.0g/L of cells and 2.2ng/mL of mGM-CSF were obtained (11.0g/L and 2.4ng/mL, respectively in shake flasks). Although the lag period was prolonged and mGM-CSF production was lowered by 33% in the stirred thank bioreactor as compared to the control culture, the maximum cell density was increased up to 12.0g/L due to better mixing by agitation at the higher cell density.

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References

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