Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Nitrogenase Derepression and Associated Metabolism in a Microaerophilic Cyanobacterium, Plectonema boryanum

  • Pandey, Kapil Deo (Center of Advance Study in Botany, Banaras Hindu University) ;
  • Sukla, Sarkar (Center of Advance Study in Botany, Banaras Hindu University) ;
  • Naz, Shaheen (Center of Advance Study in Botany, Banaras Hindu University) ;
  • Smita, Chaturvedi (Center of Advance Study in Botany, Banaras Hindu University) ;
  • Ajaikumar, Kashyap (Center of Advance Study in Botany, Banaras Hindu University)
  • Published : 2001.04.01
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Abstract

Nitrate grown cells of cyanobacterium Plectonema boryanum, transferred to nitrogen stress, evolved nitrogenase catalyzed $H_2$ under microaerophilic condition. Nitrogen ($N_2$) in gs phase, low light intensity, and reducing substances in incubation phase stimulated $N_2$fixation ($H_2\;evolution$). Cyanobacterium grew slowly under microaerobic condition with a low intracellular ammonia pool. Nitrogen sources (${NO_3}^-,{NH_4}^+,\;and\;CH_3NH_3$) inhibited nitrogenase and glutamine synthetase (GS) transferase activity, and methylamine behaved like an ammonical nitrogen source. Depletion of molybdenum (Mo) and addition of tungsten (W) in the incubation medium inhibited $H_2$ evolution, Cyanobacterium was able to take up nitrate and expressed nitrate reductase (NR) activity under microaerophilic condition at an extremely slow rate.

Keywords

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