Antimicrobial Effects of Ethanol Extracts of Pinus densiflora Sieb. et Zucc. on Lactic Acid Bacteria

김치 발효 미생물에 대한 소나무(Pinus densiflora Sieb. et Zucc.) 에탄올 추출물의 항균효과

  • 임용숙 (대구가톨릭대학교 식품공학과) ;
  • 박경남 (대구가톨릭대학교 식품공학과) ;
  • 배만종 (경산대학교 식품과학과) ;
  • 이신호 (대구가톨릭대학교 식품공학과)
  • Published : 2001.12.01

Abstract

This study was carried out to investigate the effect of ethanol extract of Pinus densiflora (PD) on the growth of lactic acid bacteria (LAB), A-1, B-9, K-7, M-7 isolated from kimchi. The growth of isolated LAB was inhibited significantly in the modified MRS broth containing 40 mg/mL PD ethanol extract. Ethyl acetate fraction showed the strongest antimicrobial activities against LAB strains compared to other fractions. The addition of PD ethanol extract to kimchi did not change the pH of kimchi greatly compare with the control during the fermentation for 25 days. Change of titratable acidity in control was more higher than in the PD ethanol extract added kimchi during fermentation. The growth of total bacteria and LAB was inhibited about 1 to 2 log cycle by the addition of PD ethanol extract during the kimchi fermentation for 25 days at 1$0^{\circ}C$. Sensory quality of PD ethanol extract added kimchi was lower than that of control.

김치의 선도를 유지할 수 있는 천연물의 사용 방안을 모색코져 소나무 추출물에 대한 김치 발효관련 유산균의 성장특성을 비교한 결과, 소나무 에탄을 추출물 40 mg/mL 첨가에 의해 김치에서 분리한 유산균인 A-1, B-9, K-7, M-7의 성장은 뚜렷하게 억제되었다. 특히 ehtyl acetate분획물이 항균 효과가 뚜렷하였다. 소나무 에탄올 추출물을 첨가한 김치 발효 25일 동안 대조구에 비해 pH와 산도의 변화는 크게 나타나지 않았다. 소나무 에탄을 추출물 첨가 김치의 숙성중 총균수와 유산균수는 대조구에 비해 전 숙성기간동안 1에서 2 log cyc1e 억제되는 경향을 나타내었다. 소나무 에탄을 추출물 첨가에 의해 김치의 숙성은 액제되는 경향을 나타내었다.

Keywords

References

  1. J. Food Sci. Technol. v.CUTH9 Changes in enzymes activities and growth of lactic acid bacteria in pine needle added kimchi during fermentation Oh. Y.A.;Choi. K.H.;Kim. S.D.
  2. J. Food Sci. Technol. v.CUTH9 Quality of pine needle added kimchi Oh. Y.A.;Sae. K.Y.;Kim. S.D.
  3. J. Korean Soc. Agric. Chem. Biotechnol. v.37 Isolation of 4-hydroxy-5-methyl-3[2H]-furanone from pine needles as an antioxidative principle Boo. Y.C.;Jean. C.O.;Oh. J.Y.
  4. J. Korean Soc. Food Sci. Nutr. v.25 Development of the health foods containing the extrcat from pinus strobus leave Lee. Y.H.;Shin. Y.M.;Cha. S.H.;Chio. Y.S.;Lee. S.Y.
  5. J. Korean Soc. Food Sci. Nutr. v.26 Changes on the components of dongchimi added ginseng and pine needle Kim. I.K.;Shin. S.R.;Chung. J.H.;Kim. K.S.
  6. J. Korean Soc. Food Sci. Nutr. v.27 Changes in enzyme activities and polpulation of lactic acid bacteria during the kimchi fermentation supplemented with water wxtract of pine needle Oh. Y.A.;Choi. K.H.;Kim. S.D.
  7. J. Korean Soc. Food Sci. Nutr. v.25 Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet Kang. Y.H.;Park. Y.K.;Ha. Y.;Moon. K.D.
  8. Korean J. Food Sci. Technol. v.27 Studies on the physiological functionality of pine needle and mugwort extracts Kang. Y.H.;Park. Y.K.;Oh. S.R.;Moon. K.D.
  9. 과학기술처 제1차년도 연차보고서 전통발효 식품의 과학화 연구(김치의 보존성 증대에 관한 연구) 김순동;이신호;노홍균
  10. J. Korean Soc. Food Sci. Nutr. v.27 Effect of hot water extracts of Salvia miltiorrhiza, Prunus persoca stokes, Angelica gigas Nakai and Pinus strobus on lipid oxidation Kim. S.M.;Cho. Y.S.;Kim. E.J.;Bae. M.J.;Han. J.P.;Lee. S.H.;Sung. S.K.
  11. J. Korean Soc. Food Sci. Nutr. v.16 Storage of salted Chinese cabbages for kimchi I, Physicochemical and microbial changes during salting of Chinese cabbages Kim. J.M.;Kim. I.S.;Yang. H.C.
  12. J. Korean Soc. Agric. Chem. Biotechnol. v.24 The change of vitamin C content and effect of galacturonic acid addition during kimchi fermentation Lee. J.W.;Lee. T.Y.
  13. Research Bulletin of Kimchi Science and Technology v.1 Critical review on biochemical characteristics of kimchi Cheigh. H.S.
  14. J. Korean Soc. Agric. Chem. Biotechnol. v.26 Microbial inhibition of lactic strains isolated from kimchi. park. Y.H.;Kwon. J.J.;Jo. D.H.;Kim. S.I.
  15. J. Korean Soc. Food Sci. Nutr. v.27 The mixed of Salvia miltiorrhiza and Glycyrrhiza uralensis on the shelf of kimchi. Lee. S.H.;Cho. O.K.;Park. N.Y.
  16. Kor. J. Appl. Microbiol. Biotechnol. v.25 Antimicrobial effects of Schizandra chinensis extract against Listeria monocytogenes Lee. S.H.;Yim. Y.S.
  17. Collins and lyne's microbiological methods (6th ed) Coolins. C.H.;Lyne. P.M.
  18. Compendium of methods for the microbiological examination of foods (3rd ed) Vanderazant. C.;Splittstoesser. D.F.
  19. Korean J. Food Sci. Technol. v.32 The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinesis Ruprecht (Omija) seed Jung. G.T.;Ju. I.O.;Choi. J.S.;Hong. J.S.
  20. Kor. J. Appl. Microbiol. Biotechnol. v.25 Effect of omija (Schizandra chin ensis) extract on the growth of lactic acid bacteria isolated from kimchi Lee. S.H.;Yim. Y.K.
  21. Kor. J. Appl. Microbiol. Biotechnol. v.25 Effect of Schizandra chinesis (omija) extract on the fermentation of kimchi. Lee. S.H.;Kim. Y.K.;Choi. W.J.
  22. Korean J. Food Sci. Technol. v.31 Effect of Casealpina sappan L. and Lithospormum erythrorhizon extract mixture and crab shell onthe fermentation of kimchi Lee. S.H.;Park. K.N.;Lim. Y.S.
  23. J. Food Sci. Technol. v.CUTH9 Fermentation of kimchi with decontaminated sub-ingredients Park. I.K.;Ku. Y.s.;Kim. S.D.