Generation of Baculovirus Expression Vector Using Detective Autographa California Nuclear Polyhedrosis Virus Genome Maintained in Escherichia coli for $Occ^{+}$ Virus Production

  • Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National Univeristy) ;
  • Chang, Jin-Hee (School of Agricultural Biotechnology, Seoul National Univeristy) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National Univeristy) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2001.06.01

초록

We have generated a novel baculovirus genome which can be maintained in Escherichia coli that facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. To make $Occ^{+}$ recombinant expression vectors, polyhedrin gene under the control of p10 promoter was inserted to bAcGOZA and this genome was designated bApGOZA. As in bAcGOZA, bApGOZA lacks a portion of the essential ORF1629 gene, but includes a mini-F replicon and selectable kanamycin-resistance marker, This occasion-producing activity of bApGOZA can be used very conveniently for its oral infectivity to insect larvae in mass production of foreign protein and insecticides.

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