한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

가시오갈피 추출물의 항돌연변이 효과

Antimutagenic Effect of Extract of Eleutherococcus senticosus Maxim

  • 박정섭 (우석대학교 생명공학부) ;
  • 오찬호 (우석대학교 생명공학부) ;
  • 고하영 (우석대학교 식품영양 식품공학부) ;
  • 최동성 (우석대학교 생명공학부)
  • Park, Jeong-Seob (Division of Bioscience and Biotechnology, Woosuk University) ;
  • Oh, Chan-Ho (Division of Bioscience and Biotechnology, Woosuk University) ;
  • Koh, Ha-Young (Division of Food Science and Nutrition, Woosuk University) ;
  • Choi, Dong-Seong (Division of Bioscience and Biotechnology, Woosuk University)
  • 발행 : 2002.12.01
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초록

가시오갈피 뿌리, 줄기, 잎의 80% 메탄올로 추출물과 용매 분획물들의 항돌연변이 효과를 조사하기 위하여 2-AF와 Trp-P-1을 사용하여 Ames test로 검토하였다. 가시오갈피 메탄올 추출물의 돌연변이원성은 없었으며, 틀변경 변이주인 S. typhimurium TA98에서는 $500\;{\mu}g/plate$ 농도에서 모두 60% 이상의 돌연변이 억제효과를 나타내었고, S. typhimurium TA100에서는 억제효과를 거의 나타내지 않았다. 이 결과로부터 가시오갈피 메탄올 추출물이 틀변경 돌연변이에 대해 강한 억제효과를 갖는다는 것이 시사되었다. 메탄올 추출물의 분획물중 클로로포름 층이 강한 항돌연변이원 효과를 나타내었고, 부탄올과 물 층에서는 억제효과가 거의 없었다. 뿌리, 줄기, 잎의 클로로포름 층은 S. typhimurium TA98에서 2-AF를 돌연변이원으로 사용했을 경우 $300\;{\mu}g/plate$ 농도에서 각각 93.6, 76.2, 68.48%의 억제효과를 나타내었고, Trp-P-1을 돌연변이원으로 사용했을 경우 각각 87.7, 72.5, 68.8%의 억제효과를 나타내었으며, 모두 용량 의존적으로 항돌연변이 효과를 나타내었다.

Antimutagenic effect of Eleutherococcus senticosus Maxim. on the mutagenicity induced by 2-AF and Trp-P-1 was studied using the Ames test with Salmonella typhimurium TA98 and TA100. In S. typhimurium TA98, the methanol extract $(500\;{\mu}g/plate)$ of root, stem, and leaf of E. senticosus showed inhibitory effects of 72.8, 70.0, and 78.7% on the mutagenicity induced by 2-AF, and 69.2, 64.9, and 59.4% by Trp-P-1, respectively, whereas none was observed in S. typhimurium TA100. These results suggest that the methanol extract of E. senticosus inhibits a frame shift mutation. And then the methanol extract further fractionated by chloroform, butanol, and water. The chloroform fractions of root, stem, and leaf showed strong antimutagenic effects induced by 2-AF and Trp-P-1 in S. typhimurium TA98, whereas none was observed in the butanol and aqueous fractions. The chloroform fractions of root, stem, and leaf showed antimutagenic effects of $13{\sim]92%$ in a dose-dependent manner.

키워드

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