Micropropagation of Kalopanax $pictus(T_{HUNB}.)\;N_{AKAI}$ by bud cultrue

  • Kim, Bong-Kyu (Department of Forest Resources, Konkuk University) ;
  • Yi, Yong-Sub (Biotechnology Division, Korea Forest Research Institute) ;
  • Ahn, Joong-Hoon (Department of Forest Resources, Konkuk University)
  • Published : 2002.11.30

Abstract

Several plant growth regulators were examined for on their effect on the in vitro propagation of Kalopanax pictus$(T_{HUNB}.)$ $(N_{AKAI}.)$ on WPM medium. Among the cytokinins tested, BA at $13.32\;{\mu}M$ appeared to be the most effective for multiple axillary shoot formation. Although the addition of $2.89\;{\mu}M\;GA_3$ promoted stem elongation, it produced morphologically abnormal leaves and stems. For rooting of the shoots, $4.9\;{\mu}M$ IBA seemed to be more effective than $2.69\;{\mu}M$ NAA. When the regenerated plants were transferred on artificial mixture containing vermiculite and peat-moss (1 : 1, v/v), 81% of them survived and grew normally.

Keywords

References

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