Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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THE EFFECT OF HYALURONIC ACID ON XENOGRAFT IN RAT CALVARIAL DEFECT

백서 두개부 결손부에 이식된 이종골 치유과정에 히알루론산이 미치는 영향에 관한 연구

  • Jo, I-Su (Department of Oral Maxillofacial Surgery, School of Dentistry, Won-kwang University) ;
  • Min, Seung-Ki (Department of Oral Maxillofacial Surgery, School of Dentistry, Won-kwang University)
  • 조이수 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 민승기 (원광대학교 치과대학 구강악안면외과학교실)
  • Published : 2002.06.30
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Abstract

The purpose of this study was to evaluate the tissue response in various bone grafting materials, especially xenogenous bone materials in vivo, compare of bone formation capacity of various bone grafting materials on rat skull defects and evaluate the effect of Hyaluronic acid on healing of human Demineralized Freezed Dried Bone allogenous graft (DFDBA) materials in rat calvarial defects. 30 Sprague-Dawly rats were divided into 4 groups. $7{\times}7mm$ size bony defect were artificially prepared in the calvaria (both parietal bone) of all 30 rats and follwed group grafting of autogenous bone graft on right side and allogenic DFDBA on left side bone graft (rat DFDB) in 15 control group, but in 15 experimental group, xenograft (human DFDB) on left side, hyaluronic acid treated with xenograft on right side. Sequential sacrifices was performed at 1, 2, 4, 6, 8 weeks of experiment. These specimens were stained with H&E and MT stain, and then histologic analysis under light microscope was carried out. There were inflammatory reaction in all graft material during early stage. Autogenous and Allogenous DFDBA graft group observed inflammatory reaction at 1 week. Xenograft group persistant inflammatory reaction until 4 weeks, but in HA treated xenograft group inflammatory reaction was decreased at 2 weeks. Osteoblastic activity in control group was begun at 2 week, xenograft group was delayed at 6 weeks, however HA treated xenograft group was begun at 4 weeks. At 2 week, mild osteoclastic activity were observed in all xenograft group not in concerned to HA, but there was no difference each group after 4 weeks. There are most activated angiogenesis around graft mateirals in xenograft group at 2 weeks, but in HA treated xenograft group, decreased angiogenesis was observed at same time. Bone formation and bone maturation of xenograft group, there was no difference in HA treatment, was less than control group. Fibrosis around xenograft materials were observed until 6 weeks, there was no difference between xenograft and HA treated groups.

Keywords

References

  1. Boyne PJ. : Induction of bone repair by various bone grafting materials, hard tissue growth, repair and remineralization. Ciba Found Symp. 11: 121-128, 1973
  2. Masters DH. : Implants, Bone and bone substitutes. Calif Dent Asso J. 16: 56-65, 1988
  3. Jarcho M. : Calicium phosphate ceramics as hard tissue prosthetics. Clin Orthop. 157: 259-278, 1981
  4. Rosenberg MM. : Free osseous tissue autografts as a predictable procedure. J Periodontol. 42: 195-209, 1971
  5. Hiatt WH, Schallhorn RG. : Intraoral transplants of cancellous bone and marrow in periodontal lesions. J Periodontol. 44: 194-208, 1973
  6. Ennecking WF, Morris JL. : Human autologous cortical bone transplants Clin Orthop. 87: 28-35, 1972
  7. Dragoo MR, Sullivan HC. : A Clinical and histological evaluation of autogenous iliac bone graft in humans. J Periodontol. 44: 599-613, 1973
  8. Becker W, Urist MR, Tucker LM, Becker BE, Ochsenbein C. : Human demineralized freeze- dried bone: Inadequate induced bone formation in athymic mice. A preliminary report. J Periodontol. 66: 822-828, 1995
  9. Diem CR, Bowers GM, Moffitt WC. : Bone blending ; A technique for osseous implants. J Periodontol. 43: 295-297, 1972
  10. Wagner JR. : A 3 1/2-year clinical evaluation of resorbable hydroxylapatite osteogen (HA Resorb) used for sinus lift augmentations in conjunction with the insertion of endosseous implants J Oral Implantol. 17: 152-164, 1991
  11. Wagner JR. : A Clinical and histological case study using resorbable hydroxylapatite for the repair of osseous defects prior to endosseous implants surgery. J Oral Implantol. 15: 186-192, 1989
  12. Costatino PD, Friedmand CD. : Synthetic bone graft substitutes. Otolaryngol Clin North Am. 27: 1037-1042, 1994
  13. Prokic B, Carranza FA, Kenny EB, et al. : Comparative clinical study of porous hydroxyapa tite and decalcified freeze-dried bone in human periodontal defects. J Periodontol. 61: 399-404, 1990
  14. Papay FA, Morales L, Ahmed OF, et al. : Comparison of ossification of demineralized bone, hydroxyapatite, Gelfoam, and bone wax in cranial defect repair. J Craniofac Surg. 7: 347-351, 1996
  15. Corsair A. : A clinical evaluation of resorbable hydroxylapatite for the repair of human intra-osseous defects. J Oral Implantol. 16: 125-128, 1990
  16. Shimizu Y, Sugawara H, Frusawa T, et al. : Bone remodeling with resorbable bioactive glass and hydroxyapatite. Implant Dent. 6: 269-274, 1997
  17. Buring K, Urist MR. : Effects of ionizing radiation on the bone induction principle in the matrix of bone implats. Clin Orthop. 44: 225-234, 1967
  18. Mellonig JT, Prewett AB, Moyer MP. : HIV inactivation in a bone allograft. J Periodontol. 63: 979-983, 1992
  19. Sydney N, Burwell RG. : Decalcified bone as a substrate for osteogenesis. J Bone Joint Surg. 59-B: 189-196, 1997
  20. Bright RW, Fridlander GE, Sell KW. : Tissue Bank Milit Med 147: 503-510, 1977
  21. Canalis E, McCarthy TL, Centrella M. : The role of growth factors in skeletal remodeling. Entocrinol Meta Cl: North Am. 18: 903-918, 1989
  22. JW Shin. : An experimental study on healing effect of the PDGF treated allogeneic demineralized freeze-dried bone transplanted in the calvarial defect of the rat. J Kor Oral Maxillofac Plast Recon Surg. 22: 614-624, 2000
  23. JS Doo, JK Kang, HK Yu, HS Shin. : Effect of Natural medicines on cell activity in vitro. J Kor Acad Periodontol. 27: 459-468, 1997
  24. JK Kang, HK Yu, HS Shin. : Effects of Hyaluronic acid on mineralization of periodontal ligament cells and osteoblastic cells. J Kor Acad Periodontol. 28: 475-489, 1998
  25. KS Lee, SW Hong, KT Yu, HK Yu, YC Kim, HS Shin. : Effects of isolated extracts from safflower seeds on mineralization of periodontal ligament cells and osteoblastic cells. J Kor Aca Periodontol. 28: 745-754, 1998
  26. JH Kim, SM Cheun, MY An, SK Ku, JH Lee, MS Cha, KD Moon. : Effects of safflower seed feeding on bone tissue during rib fracture healing time in vivo. J Kor Acad Food Nutr. 27: 698-704, 1998
  27. DH Yoon, SC Lee, MY Kim, YC Kim, HK Yu, JH Kim, HS Shin. : Effects of safflower seed extract on osteoblastic activity and bone regeneration. J Kor Aca Periodontol. 28: 769-784, 1998
  28. Meyer K, Palmer JW. : The polysaccharide of the vitreous humor. J Biol Chem. 107: 629-634, 1943
  29. Kvist TN, Finnegan CU. : Distribution of glycosaminoglycans in axil region of developing chick embryo. I. Histochemical analysis. J Exp Zool. 175 : 221-240, 1970
  30. Toole BP, Gross J. : The extracellular matrix of the regenerating newt limb; synthesis and removal of hyaluronate prior to differentiation. Dev Biol. 26: 28-35, 1971
  31. Maurer PH, Hudack SS. : The isolation of hyaluronic acid from callus tissue of early healing. Arch Biochem Biophys. 38:49-53, 1952
  32. Iwata H, Urist MR. : Hyaluronic acid production and removal during bone morphogenesis in implants of bone matrix in rats. Clin Orthop Rel Res 90: 236-245, 1973
  33. Urist MR: Biochemistry of calcification. In: Bourne GH (ed) The biochemistry and physiology of bone. Academic Prss, New York, pp 1- 59, 1976
  34. Sanberg MMHT and Vuorio EI. : Gene expression during bone repair. Clin Ortho Rel Res 289: 292-312, 1993
  35. Pilloni A, Bernard GW. : The effect of hyaluronan an mouse intramembranous osteogenesis in vitro. Cell Tissue Res. 294: 323-333, 1998
  36. Liu SS, Thompson AY, Heidaran MA, Poser JW, Spiro RC. : An osteoconductive collagen/hyaluronate matrix for bone regernation. Biomaterials 20: 1097-1108, 1999
  37. YM Cho. : The effect of hyaluronic acid on mouse calvalia preosteoblasts osteogenesis In Vitro
  38. Glowacki J, Mulliken JB. : Induced osteogenesis for repair and construction in the craniofacial region. Plast Reconstr Surg. 65: 553-560, 1980
  39. Yim CJ. : Oral and maxillofacial reconstruction with bone allograft. J Kor Acad Maxillofac Plast Reconstr Surg. 56: 706-714, 1998
  40. Glowacki J, Mulliken JB. : Demineralized implants Clin Plast Surg. 12: 233-241, 1985
  41. Nevins M, Kirker-Head C, Nevins M. et al. : Bone formation in the goat maxillary sinus induced by absorbable collagen sponge implants impregnated with recombinant human bone morphogenetic protein-2. Int J Perio Rest Dent. 16: 8-19, 1996
  42. Moy PK, Lundgren S, Holmes R. : Maxillary sinus augmentation: Histormorphometric analysis og graft materials for maxillary sinus floor augmentation. J Oral Maxillofac Surg. 55, 857-862, 1993
  43. Hirsch JM, Ericsson I. : Maxillary sinus augmentation using mandibular bone grafts and simultaneous installation of implants. Clin Oral Implant Res. 2: 91-96, 1991
  44. Smiler DG. : Surgical solutions to prosthetic problems. Steri-Oss Mongraph, August 1993, pp 44-49
  45. Lazzara RJ. : The sinus elevation procedure in endosseous implant therapy. Curr Opin Periodotol. 3: 178-183, 1996
  46. Wheeler SL. : Sinus Augmentation for Dental implants: The use of Alloplastic materials J Oral Maxillofac Surg. 55, 1287-1293, 1997
  47. Urist MR. : Bone. : Formation by autoinduction. Science 150: 893-899, 1965
  48. Thielemann FW, Spaeth G, Veihelmann D, Schmidt K. : Osteoinduction. Part I: Test model and comparative long term observation of allogenic and xenogeneic matrix implants. Arch Orthop Trauma Surg. 99 : 217-222, 1982
  49. Manilay JO, Sykes M. : Natural killer cells and their role in graft rejection. Current Opinion Immulology. 10: 532-538, 1998
  50. Urist MR, Silverman BF, Buring K, Dubuc FL, Rosenberg JM. : The bone induction principle. Clin Orthop Rel Res. 53: 243-283, 1967
  51. Urist MR, Dowell TA, Hay PH, Strates BS. : Inductive substrates for bone formation. Clin Orthop Rel Res. 59: 59-96, 1968
  52. Huggins C, Wiseman S, Reddi AH. : Transformation of fibroblasts by allogeneic and xenogeneic transplants of demineralized tooth and bone. J Exp Med. 132: 1250-1258, 1970
  53. Salama R, Burwellg, Dickson IR. : Recombined grafts of bone and marrow: the beneficial effect upon osteogenesis of impregnating xenograft(heterograft) bone with autologous red marrow. J Bone Joint Surgery. 55B: 402-417, 1973
  54. Aspenberg P, Thorngren KG, Lohmander LS. : Rabbit bone matrix induceds bone formation in the athymic rat. ACTA Orthop Scand. 59: 276-278, 1988
  55. Ripamonti U, Magan A, Ma S, Van Den Heever B, Moehl T, Reddi AH. : Xenogeneic osteogenin, a bone morphogenetic protein and demineralized bone matrices, including human, induced bone differentiation in athymic rats and baboons. Matrix. 11: 404-411, 1991
  56. Thielemann FW Schmidt K, Koslowski L. : Osteoinduction II; Purification of the osteoindu- ctive activities of bone matrix. Arch Orthop Traumat Surg. 100; 73-78, 1982
  57. Aldinger G, Herr G, Kusswetter W, Reiss HJ, Thielemann FW, Holz U. : Bone morphogenetic protein: a review. Int Orthop. 15: 169-177, 1991
  58. Richardson CR, Mellonig JT, Brunsvold MA, McDonnell HT, Cochran DL. : Clinical evaluation of Bio-Oss� : a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Clin Periodontol. 26: 421-428, 1999
  59. Valdre G, Mongiorgi R, Ferrieri P, Corvo G, Cattaneo V, Tartaro GP. : Scanning electron microscopy(SEM) and microanalysis(EDS) applied to the study of biomaterials for dental use. Miberva Stomatol. 44: 55-68, 1995
  60. Kim YK, Kim SG, Lee JG, Lee MH, Cho JO. : An experimental study on the healing process after the implantation of various bon substitutes in rats. J Kor Oral Maxillofac Surg. 27: 15-21, 2001
  61. Viljanen VV, Gao TJ, Lindholm TC, Lindholm TS, Kommonen B. : Xenogeneic mouse bone morphogenetic protein(mBMP)- induced repair of critical-size skull defect in sheep. Int J Oral Maxillofac Surg. 25: 217-222, 1996
  62. Viljanen VV, Lindholm TS. : Comparison of native xenogeneic and allogeneic bone morphogenetic proteins in the sheep skull defect assay model. Ann Chir Gynaecol. 86: 255-259, 1997
  63. Cho KS, Choi SH, Han JH, Chai JK, Wikesjo UME, Kim CK. : Alveolar bone formation at dental implant dehesience defects following guided bone regeneration and xenogeneic freeze-dried demineralized bone matrix. Clin Oral Impl Res. 9; 419-428, 1998
  64. Tuominen T, Jamsa T, Tuukkannen J, Marttinen A, Lindholm TS, Jalovaara P. : Bovine bone implant with bovine bone morphogenetic protein in healing a canine ulnar defect. Inter Orthop. 25:5-8, 2001