KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Wastewater Treatment and Microbial Structure Analysis by Fluorescence In Situ Hydridizationin a Biofilm Reactor

생물막 반응키에서의 폐수 처리 및 Fluorescence In Situ Hybridization에 의한 복합 미생물계 구조 해석

  • Kim, Dong-Jin (Department of Environmental Systems Engineering, Hallym University) ;
  • Han, Dong-Woo (Department of Environmental Systems Engineering, Hallym University) ;
  • Lee, Soo-Choul (Research Institute of Bioscience and Biotechnology, Dbio, Inc.) ;
  • Park, Byeong-Gon (Research Institute of Bioscience and Biotechnology, Dbio, Inc.) ;
  • Kwon, Il (Research Institute of Bioscience and Biotechnology, Dbio, Inc.) ;
  • Sung, Chang-Keun (Department of Food Science and Technology, Chungnam National University) ;
  • Park, Wan-Cheol (Water Environment Research Center, KIST)
  • 김동진 (한림대학교 환경시스템공학과) ;
  • 한동우 (한림대학교 환경시스템공학과) ;
  • 이수철 ((주)대덕바이오 부설 생물공학연구소) ;
  • 박병곤 ((주)대덕바이오 부설 생물공학연구소) ;
  • 권일 ((주)대덕바이오 부설 생물공학연구소) ;
  • 성창근 (충남대학교 식품공학과) ;
  • 박완철 (한국과학기술연구원 수질환경연구센터)
  • Published : 2002.02.01
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Abstract

Laboratory scale aerobicfanaerobic biofilm reactor was used for simultaneous and stable removal of organics, N and P components to investigate optimum design and operation parameters and to analyze the microbial distribution and consortium structure of nitrification and denitrification bacteria in aerobic and anaerobic biofilm systems. The biofilm reactor was successfully operated for 143 days to show $COD_{cr},\;BOD_5$, SS removal efficiencies of 88, 88, and 97%, respectively. During the experiment period, almost complete nitrification efficiency of 96% was achieved. Denitrification efficiency was about 45% without addition of any external carbon sources. In case of total phosphorus removal, 74% of the inlet phosphorus was removed. Fluorescence in situ hybridization (FISH) results showed that most of the ammonia oxidizing bacteria in the aerobic nitrification zone was found to be Nitrosomonas species while Nitrospira was the representative nitrite oxidizing bacteria. For the denitrification, Rhodobacter, Rhodovulum, Roseebacter and Paracouus were the dominant denitrification bacteria which was 10 to 20% of the total bacteria in numbers.

유기물 제거뿐만 아니라 안정적으로 질소와 인의 동시 제거를 위한 순환식 생물막 반응기를 제작, 운전하여 최적의 운전 인자를 도출하고, 질소 제거의 텃 번째 단계인 질산화 및 뒤이은 탈질에 관여하는 미생물들의 군집 구조 분석을 수행하였다. 유기물 제거와 질소와 인의 동시 제거를 위한 순환식 생물막 반응기는 143일 동안 운전되었다. 이 결과 $COD_{cr},\;BOD_5$, SS의 경우 각각 88, 88, 97%의 평균 제거효율을 보였다 이 기간 중 질산화율은 약 96% 정도로 유입 ${{NH_4}^{+}}_{-}N$의 대부분이 제거됨을 보였다. 하지만 탈질율은 평균 45% 정도로 나타났다. 반응기로 유입되는 총 인의 경우 약 44%가 제거되었다. 질소제거의 첫 번째 단계인 질산화가 일어나는 호기성 반응조 내 질산화 미생물의 경우 FISH 관찰 결과, 주요 암모니아 산화균 및 아질산 산화균으로는 Nitrosomonas spp.와 Nitrospira sap.가 관찰되었다. 또한 탈질 반응이 일어나는 준혐기성 반응조에서는 Rhodobacter, Rhodovulum, Roseebacter 그리고 Paracoccus 속에 속하는 탈질 미생물들이 전체 미생물의 약 10~20% 정도를 차지하며 분포하였다.

Keywords

References

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