Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)

  • Ock, S.A. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kwack, D.O. (Department of Biology, Gyengsang National University) ;
  • Cho, S.R. (Division of Science Education, Gyengsang National University) ;
  • Cho, S.K. (Applied Animal Science, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yeao, E.H. (Applied Animal Science, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yoo, J.G. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Y.R. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, H.J. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Choe, S.Y. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Rho, G.J. (Applied Animal Sciences, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Published : 2002.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.

Keywords

References

  1. Calvin HI and Bedford JM. 1971. Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during maturation in the epididymis. J. Reprod. Fertil., (Suppl. 13): 65-75
  2. Campbell KHS, Ritchie WA and Wilmut I. 1993 Nuclear-cytoplasmic interactions during the first cell cycle of nuclear transfer reconstructed bovine embryos: Implications for deoxyribonucleic acid replication and development. Biol. Reprod., 49: 933-942 https://doi.org/10.1095/biolreprod49.5.933
  3. Chen SH and Seidel GE Jr. 1997. Effects of oocytes activation and treatment of spermatozoa on embryonic development following intracytoplasmic sperm injection in cattle. Theriogenology, 48:1265-1273 https://doi.org/10.1016/S0093-691X(97)00187-8
  4. De la Fuente P and King WA. 1998. Developmental consequences of karyokinesis without cytokiness during the first meiotic cell cycle of bovine parthenotes. Biol. Reprod., 58: 952-962 https://doi.org/10.1095/biolreprod58.4.952
  5. Fukui Y, Sawai K, Furudate M, Sato N, Iwazumi Y and Ohasaki K. 1992. Parthenogenetic development of bovine oocytes treated with ethanol and cyochalasin B after in vitro maturation. Mol. Reprod. Dev., 33:357-362 https://doi.org/10.1002/mrd.1080330318
  6. Gomez MC, Catt JW, Evans G and Maxwell WMC. 1998. Sheep oocyte activation after intracytoplasmic sperm injection (ICSl). Reprod. Fertil. Dev., 40:43-52
  7. Jones KT, Soller C and Cannell MB. 1998. The passage of $Ca^{2+}$ and fluorescent markers between the sperm and egg after fusion in the mouse. Development, 125:4627-4635
  8. Kaufman MH. 1983. Methodology: In vitro and in vivo techniques. In MH Kauman (ed) : 'Early Mammalian Development : Parthenogenetic Studies'. Cambridge University Press, 20-26
  9. Keefer CL, Younis AI and Brackett BG. 1990. Cleavage development of bovine oocyte fertilized by sperm injection. Mol. Reprod. Dev., 25: 281-285 https://doi.org/10.1002/mrd.1080250310
  10. Kimura Y and Yanagimachi R. 1995. Intracytoplasmic sperm injection in the mouse. Biol. Reprod., 52:709-720 https://doi.org/10.1095/biolreprod52.4.709
  11. King WA, Linares T, Gustavsson I and Bane A. 1979. A method for preparation of chromosomes from bovine zygotes and blastocysta. Vet. Sci. Comm., 3:51-56 https://doi.org/10.1007/BF02268951
  12. Kubiak JZ. 1989. Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest. Dev. Biol., 136:537-545 https://doi.org/10.1016/0012-1606(89)90279-0
  13. Minamihashi A, Waston AJ, Waston PH, Church RB and Schurltz GA. 1993. Bwine parthenogenetic blastocysts following in vitro maturation and oocyte activation with ethanol. Theriogenology, 40:63-76 https://doi.org/10.1016/0093-691X(93)90341-2
  14. Nagai T. 1987. Parthenogenetic activation of cattle follicular oocytes in vitro with ethanol. Gamete Res., 16:243-249 https://doi.org/10.1002/mrd.1120160306
  15. Navara CS, First NL and Schatten G. 1994. Microtubule organization in the cow during fertilization, polyspermy, Parthenogenesis, and nuclear transfer: the role of the sperm aster. Dev. Biol., 162:29-40 https://doi.org/10.1006/dbio.1994.1064
  16. Perreault SD and Zirkin BR. 1982. Sperm nuclear decondensation in mammals : role of sperm-associated proteniase in vivo. J. Exp. Zool., 24:253-257
  17. Perreault SD, Barbee RR, Elstein KH, Zucker RM and Keefer CL. 1988. Interspecies difference in the stability of mammalian sperm nuclei assessed in vivo by sperm microinjection and in vitro by flow cytometry. Biol. Reprod., 39:157-167 https://doi.org/10.1095/biolreprod39.1.157
  18. Powell JW and Barnes FL. 1992. The kinetic of oocytes activation and polar body formation in bovine embryo clones. Mol. Reprod. Dev., 33:53-58 https://doi.org/10.1002/mrd.1080330108
  19. Prochazka R, Dumford R, Fiser PS and Marcus GJ. 1993. Parthenogenetic development of activated in vitro matured bovine oocytes. Theriogenology, 39:1025-1032 https://doi.org/10.1016/0093-691X(93)90003-N
  20. Qian XQ, Inagaki H, Sasada H and Sugawara S. 1996. Decondensation and male pronuclear formation in bovine oocytes after microinjection of bovine sperm pretreated with disulfide bond reducing agent. J. Mammal. Ova. Res., 13:118-121 https://doi.org/10.1274/jmor.13.118
  21. Rho GJ, Wu B, Kawarsky S, Leibo SP and Betteridge KJ. 1998a. Activation regimens to prepare bovine oocytes for intracytoplasmic sperm injection. Mol. Reprod. Dev., 50:485-492 https://doi.org/10.1002/(SICI)1098-2795(199808)50:4<485::AID-MRD12>3.0.CO;2-1
  22. Rho GJ, Kawarsky S, Johnson WH, Kochar K and Betteridge KJ. 1998b. Sperm and oocytes treatment to improve the formation of male and female pronuclei and subsequent development following intracytoplasmic sperm injection into bovine oocytes. Biol. Reprod., 59:918-924 https://doi.org/10.1095/biolreprod59.4.918
  23. Rho GJ, Hahnel AC and Betteridge KJ. 2001. Comparisons of oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro. Theriogenology, 2001. 56:503-516 https://doi.org/10.1016/S0093-691X(01)00581-7
  24. Rosenkrans CF, Zeng GQ, MCNamara, GT, Schoff PK and First NL. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod., 49:459-62 https://doi.org/10.1095/biolreprod49.3.459
  25. Rieger D, Grisart B, Semple E, Van Langendonckt A, Betterdge KJ and Dessy F. 1995. Comparison of effects of oviductal cell co-culture and oviductal cell-conditioned medium on the development and metabolic activity of cattle embryos. J. Reprod. Fertil., 105:91-98 https://doi.org/10.1530/jrf.0.1050091
  26. Stice SL and Robl JM. 1990. Activation of mammalian oocytes by a factor obtained from rabbit sperm. Mol. Reprod. Dev., 25:272-280 https://doi.org/10.1002/mrd.1080250309
  27. Stice SL, Keefer CL, Matthews L. 1994. Bovine nuclear transfer embryos: Oocyte activation prior blastomere fusion. Mol. Reprod. Dev., 38:61-68 https://doi.org/10.1002/mrd.1080380111
  28. Susko-Parrish JL, Leibfried-Rutledge ML, Northey DL, Schultzkus V and First NL. 1994. Inhibition of protein kinase after induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion. Dev. Bio., 166:729-739 https://doi.org/10.1006/dbio.1994.1351
  29. Szollosi MS, Kubiak JZ, Debey P, de Pennart H, Szollosi D and Maro B. 1993. Inhibition of protein kinases by 6-dimethylaminopurine accelerates the transition to interphase in activated mouse oocytes. J. Cell. Sci., 104:861-872
  30. Takano H, Koyoma K, Kozai C, Kato Y and Tsunoda Y. 1993. Effect of aging of recipient oocytes on the development of bovine nuclear transfer embryos in vivo. Theriogenology, 39:909-917 https://doi.org/10.1016/0093-691X(93)90428-8
  31. Tombes RM, Simerely C, Borisy GG and Schatten G. 1992. Meioses, egg activation, and nuclear envelope breakdown are differently reliant on $Ca^{2+}$ independent in the mouse. J. Cell. Biol., 117:799-811 https://doi.org/10.1083/jcb.117.4.799
  32. Van Steirteghem AC. Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde MP, Van Assche E and Devroey P. 1993. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum. Reprod., 8(7): 1055-1060 https://doi.org/10.1093/oxfordjournals.humrep.a138191
  33. Ware C, Barnes F, Maiki-Lauria M and First NL. 1989. Age dependence of bovine oocyte activation. Gamete Res., 22:265-275 https://doi.org/10.1002/mrd.1120220304
  34. Yang X, Jiang S, Farrell P, Foote RH and McGreath AB. 1993. Nuclear transfer cattle: Effect of nuclear donor cell, cytoplast age, co-culture, and embryo transfer. Mol. Reprod. Dev., 35:29-36 https://doi.org/10.1002/mrd.1080350106
  35. Yang X, Presicce GA, Moraghan L, Jiang S and Foote RH. 1994. Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes. Theriogenology, 41:395-403 https://doi.org/10.1016/0093-691X(94)90075-T
  36. Younis AI, Keefer CL and Brackett BG. 1989. Fertilization of bovine oocytes by sperm injection. Theriogenology, 31:276 (Abstr.) https://doi.org/10.1016/0093-691X(89)90684-5
  37. Zirkin BR, Chang TSK and Heapes J. 1980. Involvement of an acrosin-like proteinase in the sulfhydryl-induced degradation of rabbit sperm nuclear protamine. J. Cell Biol., 85:116-121 https://doi.org/10.1083/jcb.85.1.116