Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Characteristics of High Acidity Producing Acetic Acid Bacteria Isolated from Industrial Vinegar Fermentation

발효 중인 양초식초에서 분리한 고산도 초산균의 생육특성

  • 박미화 (경상대학교 응용생명과학부) ;
  • 류동규 (경상대학교 응용생명과학부) ;
  • 류충호 (경상대학교 응용생명과학부)
  • Published : 2002.06.01
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Abstract

Acetic acid bacteria have been isolated from running high-acid vinegar fermentation. The color of the isolated colony was beige-yellowish. Isolated cell was rod-shaped, small, pale, absolutely aerobic and gram-negative. Microscopically the cells appeared as non-motile and non-flagellated, preferentially occuring in pairs. The optimum temperature and pH for culture were 30$\^{C}$ and 2.7, respectively. The strain was able to grow in the presence of acetic acid, ethanol and glucose. Ethanol was oxidized to acetic acid which was not oxidized any more. The isolated strain utilized glucose, fructose, maltose, sucrose, mannitol and sorbitol as carbon source. Cellulose formation was not detected on bouillon. The DNA (G +C) content of isolated strain was determined to be 56.2 mol%. The strain isolated from industrial vinegar fermentation was identified as Gluconacetobacter europaeus.

양조식초발효탱크에서 9% 이상의 고산도 초산 생성균을 분리하여 그 균주의 특성을 규명하였다. 분리된 초산균은 AE중층 한천배지에서 연한 베이지색의 colony를 형성하였고 전자현미경 관찰 결과 간균, Gram음성의 세균으로 flag-ella가 관찰되지 않아 운동성이 없을 것으로 추정된다. 배양액을 검경한 결과 분리균은 단일균 또는 2개가 짝을 지어 있는 쌍구균임을 알 수 있었다. 이 분리균주의 최적 생육온도와 pH는 3$0^{\circ}C$와 2.7이었다. 4~8% 초산을 함유한 AE배지에서 잘 생육하였고 pH 2.5의 초산을 첨가한 배지에서 잘 생육하였으나 초산을 첨가하지 않은 AE배지에서는 생육하지 않았다. Glucose를 이용하여 2-, 5-ketogluconate를 형성하였다. Ethanol 존재 하에서는 잘 생육하였으나 lactate 존재 하에서는 생육이 억제되었으며 cellulose를 생성하지 않았다. HPLC로 분석한 DNA 염기 중의 G와 C 함량(mol%)은 56.2 mol%이었다. 분리된 초산균은 탄소원으로 fructose, maltose, sucrose, mannitol, sorbitol을 첨가한 배지에서 잘 생육하였고 AE액체 배지 에 ethanol을 첨가하였을 경우와 propanol를 첨가한 경우 모두 잘 생육하였다. 생화학적 특성 및 G와 C함량을 다른 표준균주들과 비교한 결과 분리된 균주는 Gluconacetobacter europeus임을 확인할 수 있었다.

Keywords

References

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