Abstract
As extraction solvent, ethanol fraction was revealed the highest anti-microbial activities. The butanol, ethyl acetate and chloroform fractions were also revealed anti-microbial activities with less extent compared to ethanol fraction. All fractions exhibited to inhibition of bacterial growth regardless gram positive and negative, yeast and fungi, however, was not exhibited effectively to their growth. In the inhibition activities against E. coli, the complete inhibition concentration of Yecheon propolis were at 0.40mg/ml, respectively. Complete inhibition concentration of Youngwol was revealed at 0.25mg/ml after 12 hours incubation. In the inhibition activities against P. aeruginosa, the complete inhibition concentration of Youngwol propolis wat at 0.20mg/ml. Yecheon propolis was reveraled the inhibition at 0.20mg/ml after 12 hours incubation. In the inhibition activities against S. typhimurium, the complete inhibition concentration of Youngwol propolis was at 0.20 and 0.25mg/ml and Yecheon was 0.40mg/ml. The Minimum Inhibitory Concentration (MIC) of ethanol extraction fraction of Yecheon propolis to P. aeruginosa, S. aureus, and S. typhimurium were > 0.2, 0.25, 0.25 and 0.4∼>0.5mg/ml, and of Youngwol propolis to P. aeruginosa, E. coli, S. aureus, S. typhimurium, B. subtlis and C. utilis were 0.15, 0.25, 0.25, 0.3 and 0.4mg/ml, respectively.
예천산 프로폴리스는 ethanol과 butanol 분획물이 S.typhimurium과 B. subtilis에 강한 항미생물 활성을 보였으며, 용매별로는 butanol, ethyl acetate, chloroform 분획물의 순이었다. C. utilis와 A. niger에는 모든 분획물이 항균활성을 나타내지 않았다. 농도별 균생육 억제 시험 결과, E. coli의 경우 예천산은 0.4mg/ml에서 초기에 균증식이 억제되었으며 영월은 0.25mg/ml에서 12시간 이후 균증식이 완전히 억제되었다. S. aureus의 경우, 영월과 예천산 프로폴리스가 0.25와 0.30mg/ml에서 완전히 균증식을 억제시켰다. P. aeruginosa의 경우, 예천산 프로폴리스는 0.20mg/ml에서 12시간 이후 균생장 억제를 보였으며, 영월산 프로폴리스는 0.20mg/ml에서 균생장이 완전히 억제되었다. S. typhimurium의 경우, 영월산은 0.20, 0.25mg/ml에서 균증식이 거의 억제되었으며, 예천산은 0.40mg/ml에서 균증식이 거의 억제되었다. MIC는 예천산 프로폴리스가 P. aeruginosa가 >0.2mg/ml로 가장 낮았고 S. aureus 0.25mg/ml, S. typhimurium > 0.35mg/ml 순이었다. 영월산은 P. aeruginosa가 0.15mg/ml로 가장 낮았으며 E. coli, S. aureus 및 S. typhimurium이 0.25mg/ml, B. subtilis 와 C. utilis는 각각 0.3, 0.4mg/ml 이었다.
