DOI QR코드

DOI QR Code

CAPS marker에 의한 Arabidopsis의 자외선 B 감수성 유전자 지도작성

Mapping of UV-B sensitive gene in Arabidopsis by CAPS markers

  • 박홍덕 (대구가톨릭대학교 생물학과) ;
  • 김종봉 (대구가톨릭대학교 생물학과)
  • 발행 : 2002.12.01

초록

Arabidopsis thaliana columbia의 종자에 EMS를 처리하여 돌연변이체들을 만들었고 이중 UV-B에 감수성이 높은 돌연변이체를 골랐다. 이 UV-B 감수성 돌연변이체의 원인 유전자를 밝히기 위하여 교배 실험을 한 결과 이는 Mendel 유전법칙을 따르고 단일 유전자의 돌연변이에 의하여 나타나며 열성 유전을 하는 것으로 밝혀져 이 유전자를 uvs라 하였다. 염색체상의 uvs의 위치를 밝히기 위하여 CAPS maker를 이용한 연관분석을 하고자 하였고 이를 위하여 각각 maker의 primer 10종류를 제작하였다. 이를 이용, 각 PCR 산물에 대하여 uvs mutant와는 다른 제한효소 pattern를 갖는 Lansberg와 uvs mutant를 교배시켜서 얻은 것들로부터 DNA를 추출하여 PCR을 수행하였다. 이들과 자외선과의 감수성을 연관시켜 교차율을 계산한 결과 5번 염색체의 LFY3과 가장 가까웁게 연관되어 있었다.

A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.

키워드

참고문헌

  1. Current Protocols in Molecular Biology Ausubel, F. M.;Brent, R.;Kingston, R. E.;Moore, D. D.;Seidman, J. G.;Smith, J. A.;Struhl, K.
  2. Physiol. Plant. v.83 Photomorphogenic effects of UV-B radiation of hypocotyl elongation in wild type and stable-phytochromodeficient mutant seedings of cucumber Bailare, C.I.;Barnes, P.W.;Kendrick, R.E. https://doi.org/10.1111/j.1399-3054.1991.tb02483.x
  3. J. Environ. Pathol. Toxicol. Oncol. v.20 Molecular basis of UV-senstive mutant strain MBH3 of Hamophilus influenza. Rd : Identification of mutation in the urv A gene Balsara, R. D.;Joshi, V. P.
  4. Trends in Cell Biol. v.7 Lignt signal transduction in plants Barnes, S. A.;McGrath, R. B.;Chua, N.H. https://doi.org/10.1016/S0962-8924(97)10043-5
  5. Trend Genet. v.7 Recombinant inbreds for molecular mapping in maize: theoretical and practical considerations Burr, B.;Burr, F. A.
  6. Plant Physiol. v.101 Ultraviolet-induced photodegradation of cucumber(Cucumis sativus L.) microsomal and soluble protein tryptophanyl residues in vitro Caldwell, C.R.
  7. Plant cell v.6 A ray of light on DNA repair Chasan, R. https://doi.org/10.1105/tpc.6.2.159
  8. Archiv. Biochem. Biophysic. v.326 Serge Upregulation of the Drosophila 1731 retrotransposon long-terminal repeat by UV-B irradiation requires a short sequence in the U3 region Eric, F.;B.B. Martin, C. https://doi.org/10.1006/abbi.1996.0068
  9. Physiol Plant. v.92 Photooxidative stress in plants Physiol Plant Foyer, C.H.;Lelandais, M.;Kunert, K.J. https://doi.org/10.1111/j.1399-3054.1994.tb03042.x
  10. Physiol. Plant. v.58 The effects of ultraviolet-B radiation on olant competition in terrestrial ecosystems Gold, G.;Caldwell, M.M. https://doi.org/10.1111/j.1399-3054.1983.tb04205.x
  11. Plant Cell. v.6 Isolation of uvhl, an Arabidopsis mutant hypersensitive to ultraviolet light and ionizing radiation Harlow, G.R.;Jenkins, M.E.;Pittalwala, T.S.;Mount, D.W. https://doi.org/10.1105/tpc.6.2.227
  12. Photochem. Photobiol. v.4 Membrane damage in UV-irradiated lenses Hightower, K.R.;McCready, J.P.;Borchman, D.
  13. Plant journal. v.4 no.2 Procedure for mapping Arabidopsis mutations using Co-dominant ecotype-specific PCR-based markers Konieczny, A.;Ausubel, F.A.
  14. Physiol. Plant. v.93 Effects of UV-B on flavonoids, ferrulic acid, growth and photo-synthesis in barley primary leaves Liu, L.;Gitz, D.C.;McClure, J.W. https://doi.org/10.1111/j.1399-3054.1995.tb05123.x
  15. Planta. v.4 Accumulation of UV-absorbing flavonoids induced by UV-B radiation in Arabidopsis thaliana Lois, R.
  16. Stratospheric Ozone Reduction. Solar Ultraviolet Radiation and Plant Life Effects of ulreaviolet-B radiation on thegrowth and productivity of field grown soybean Lydon, J.;Teramura, A.H.;Summers, E.G.;Worrest, R.C.(ed.);Caldwell, M.M.(ed.)
  17. Research Bullertin of Cath. Univ. of Deagu v.58 Effect of Ultraviolet on the protein syntheis in Arabidopsis Kim, J. B.
  18. Nucl. Acids Res. v.23 Novel DNA binding proteins highly specific to UV_damaged DNA sequences form embryos of Drosophila melanogaster Mihoko K.;T.Toshiaki(et al.) https://doi.org/10.1093/nar/23.14.2600
  19. Photochem. Photobiol. v.48 Response differences between two soy bean cultivars with contrasting UV-B radiation sensitivities Murali, N.S.;Teramura, A.H.;Randall, S.K. https://doi.org/10.1111/j.1751-1097.1988.tb02877.x
  20. Plant Cell v.1 Restriction fragment length polymorphism linkage map of Arabidopsis thaliana Nam. H-G.;Giraudat, J.;den Boer, B.;Moonan, F.;Loos, W.D.B.M.;Hauge, B.M.;Goodman, H.M. https://doi.org/10.1105/tpc.1.7.699
  21. Photochem Photobiol. v.49 On the mechanism of photosystem Ⅱ deterioration by UV-B radiation Renger, G.;Voeiker, M.;Eckert, H.J.;Fromme, R.;Hohm-Veit, S.;Graeber, P. https://doi.org/10.1111/j.1751-1097.1989.tb04083.x
  22. Theor. Appl. Genet. v.84 Sequence-tagged site-facilitated PCR for barley genome mapping Tragoontung, S.;Kanizin, V.;Hayes, P.M.;Blake, T.K.
  23. Annual Meeting of the American society of Plant Physiologists;Plant Physiology Supplement v.105 Spectral dependence of UV-B effects on the morphology and physiology of wild type and albino mutants of Arabidopsis thaliana Wilson, M.I.;Grssnberg, B.M.
  24. Photochem. Photobiol. v.50 UV-B effects on terrestrial plants Tevini, M.;Teramura, A.H. https://doi.org/10.1111/j.1751-1097.1989.tb05552.x